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Comparison of gene expression in control and BRG1-knockdown H358 cell lines

ABSTRACT: Transcription was assessed in the human H358 NSCLC cell line after knockdown of BRG1, one of the mutually exclusive subunits of the SWI/SNF chromatin remodeling complex, by 2 different shRNAs. One biolgical replicate of each treatment; 4 technical replicates of each treatment.

ORGANISM(S): Homo sapiens

SUBMITTER: Vonn Walter

PROVIDER: E-GEOD-58542 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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BRG1/SMARCA4 inactivation promotes non-small cell lung cancer aggressiveness by altering chromatin organization.

Orvis Tess T Hepperla Austin A Walter Vonn V Song Shujie S Simon Jeremy J Parker Joel J Wilkerson Matthew D MD Desai Nisarg N Major Michael B MB Hayes D Neil DN Davis Ian J IJ Weissman Bernard B

Cancer research 20140812 22

SWI/SNF chromatin remodeling complexes regulate critical cellular processes, including cell-cycle control, programmed cell death, differentiation, genomic instability, and DNA repair. Inactivation of this class of chromatin remodeling complex has been associated with a variety of malignancies, including lung, ovarian, renal, liver, and pediatric cancers. In particular, approximately 10% of primary human lung non-small cell lung cancers (NSCLC) display attenuations in the BRG1 ATPase, a core fact ...[more]

PMID: 25115300

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