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Comparison of mouse naïve (CD44lo) CD8+ T cells sorted into the highest and lowest 20% with respect to CD5

ABSTRACT: Lymphocytes from spleen and lymph nodes of unimmunized adult C57BL/6 mice were isolated, stained with antibodies for flow cytometry, and sorted into the CD8+ CD44lo CD5hi and CD5lo pool Lymphocytes from spleen and lymph nodes of unimmunized adult C57BL/6 mice were isolated, stained with antibodies for flow cytometry, and sorted into the CD8+ CD44lo CD5hi and CD5lo pool

ORGANISM(S): Mus musculus

SUBMITTER: Stephen Jameson

PROVIDER: E-GEOD-62142 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparison of mouse naïve (CD44lo) CD8+ T cells sorted into the highest and lowest 20% with respect to CD5

Project description:Lymphocytes from spleen and lymph nodes of unimmunized adult C57BL/6 mice were isolated, stained with antibodies for flow cytometry, and sorted into the CD8+ CD44lo CD5hi and CD5lo pool

2014-10-08 | GSE62142 | GEO

Comparison of CD8+ T-cell sub-types from peripheral lymph nodes and spleen from Balb/c or C57Bl/6J SPF mice mice by transcription profiling.

Project description:Young Balb/c or C57Bl/6J SPF mice (10-12 weeks) were irradiated with 4 Gy and sacrificed 16 days after irradiation. Lymphocytes from peripheral lymph nodes and spleen were stained with TCRβ, CD8, CD44, CD49d, CD122 and a viability dye and sorted by BD Influx to Trizol LS. Each sample consisted of a pool of lymphocytes originating from 2-5 mice. Alternatively, young (7-12 weeks) and aged (12-19 months) Balb/c or C57Bl/6J mice were sacrificed and their lymphocytes were sorted following the same protocol. For each population, data were obtained from 3 independent biological triplicates.

2020-07-25 | E-MTAB-9091 | biostudies-arrayexpress

Optimized CD8 effector TL

Project description:Transcriptome analyses of naive, effector and memory CD8 TCRP1A lymphocytes expressing or not an active form of the transcription factor Stat5. TCRP1A CD8 T lymphocytes were activated by their cognate Ag for 72h to induce their differentiation in effector T cells (TCRP1A eTL 72h: 4 replicates S1, S2, S3, S4). In some samples, an active form of Stat5 was introduced (TCRP1A Stat5ca eTL 72h: 2 replicates S9, S10). These 72h activated T cells were either purified and analyzed directly (samples mentioned above) or injected in congeneic hosts and recovered more than 20 days later from the host spleen and lymph nodes: TCRP1A eTL >d20: 2 replicates– S30, S32; TCRP1A Stat5ca eTL >d20: 4 replicates S11, S12, S13, S14). Naive TCRP1A CD8 T lymphocytes (TCRP1A-naive: 4 replicates S33, S34, S35, S36) are included as controls. TCRP1A CD8 T lymphocytes were activated by anti-CD3/CD28. After 24h, an active form of Stat5 was introduced in activated cells. Culture was continued for another 48h to induce their differentiation in effector T cells. These 72h activated T cells were either directly injected in congeneic hosts and recovered more than 14 days later from the host spleen and lymph nodes: T-BetKO Stat5ca >d14: 3 replicates S39, S40, S41.

2013-09-13 | E-GEOD-26945 | biostudies-arrayexpress

Expression data from BCL6-YFP-positive Tfh cells, BCL6-YFP-negative Tfh cells, non-Tfh cells, and naïve helper T cells.

Project description:We found that a number of Tfh cells downmodulated BCL6 protein after their development, and we sought to compare the gene expression between BCL6-hi Tfh cells and BCL6-low Tfh cells. CD4+ T cells were sorted from immunized and non-immunized mice for RNA extraction and hybridization on Affymetrix microarrays. Bcl6yfp/+ OT-II cells were transferred to congenic recipient mice, and immunized with NP-OVA in CFA subcutaneously. Seven or ten days after immunization, cells were collected from draining lymph nodes, and sorted on FACSAria by the expression of CXCR5, PD-1 and BCL6-YFP. Naive CD4+ T cells were CD4+ CD44lo CD62Lhi cells from unimmunized mice.

2011-06-02 | E-GEOD-24574 | biostudies-arrayexpress

single-cell RNA sequencing of effector memory CD8+ T cells of nasal-associated lymphoid tissue (NALT), spleen, submandibular & cervical lymph nodes and blood of Epstein Barr Virus (EBV)-infected NSG mice engrafted with a human immune system (HIS mice)

Project description:To identify the difference in transcriptome of effector memory CD8+ T cells in different tissues of EBV-infected HIS mice, we sorted CD8+ CD62L- CD45RA- cells from NALT, spleen, lymph node and blood via flow cytometry-assisted sorting. We pooled the cells of three individual mice together in equal cell count to gain heterogenous samples. Using the 10X Genomics platform, we performed single-cell RNA sequencing of the cells to identify their transcriptome.

2024-08-31 | E-MTAB-13853 | biostudies-arrayexpress

CD8 CDKN2A-/- lymphocytes expressing an active form of the transcription factor Stat5

Project description:Transcriptome analyses of memory CDKN2A-/- CD8 T lymphocytes expressing an active form of the transcription factor Stat5. CDKN2A-/- CD8 T lymphocytes were activated by anti-CD3/CD28. After 40h, an active form of Stat5 was introduced in activated cells. Culture was continued for another 48h to induce their differentiation in effector T cells. These 88h activated T cells were injected in congeneic hosts and recovered 15 days later from the host spleen and lymph nodes: CDKN2A-/- Stat5ca -d15: 2 replicates– S46, S47. This study is a complement of the samples previously deposited under accession number GSE41819. Those two new samples are compared to previously deposited: - activated CD8 T lymphocytes transduced to express Stat5ca and transferred in congenic hosts: o S11 (GSM663446); o S12 (GSM663445); o S13 (GSM663444); o S14 (GSM663439). and - naive peripheral CD8 T lymphocytes : o S33 (GSM663441); o S34 (GSM663435); o S35 (GSM663449); o S36 (GSM663447).

2015-05-04 | E-GEOD-56154 | biostudies-arrayexpress

CD8+ T cells during acute viral respiratory infection are uniquely differentiated and regulated by multiple inhibitory receptors

Project description:Acute viral infection typically generates functional effector CD8+ T cells that aid in pathogen clearance. However, during acute viral lower respiratory infection (LRI), lung CD8+ T cells are functionally impaired and do not optimally control viral replication, while spleen CD8+ T cells specific for the same viral epitopes remain fully functional. To better understand the mechanisms governing lung CD8+ T cell impairment, we used flow cytometry to sort anti-viral CD8+ T cells during viral LRI. Lung and spleen cells were stained with MHC-class I tetramers representing the immunodominant anti-viral CD8+ T cell epitope. We then sorted to high purity: naïve CD8+ T cells, spleen epitope-specific CD8+ T cells, lung epitope-specific CD8+ cells and secondary infection lung epitope-specific CD8+ T cells. We then performed a genome wide transcriptional analysis of these cells to characterize the gene expression profile of lung CD8+ T cell impairment.

2015-10-01 | GSE53349 | GEO

Transcription profiling of mouseWT vs CD137L-/- GC B cells

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2009-07-24 | E-NCMF-29 | biostudies-arrayexpress

Isolation of spleen lymph during leukemia development

Project description:Here we asked whether infiltration of leukemic blasts initiated a response that could be detected in the interstitial fluid phase of the spleen in a rat model known to mimic human acute myeloid leukemia (AML). By cannulating efferent lymphatic vessels, we were able to monitor the response of the spleen microenvironment during leukemia development. Flow cytometric analysis of lymphocytes isolated from spleen lymph showed increased STAT3 and CREB signaling, and proteins related to these pathways were identified with a different profile in leukemic when compared with control spleen lymph. Additionally, SPARC-like 1 protein, recently identified as a promoter of AML cell growth and a biomarker of patient outcome, was locally produced in the spleen and upregulated in the leukemic setting. Thus, interstitial fluid, and its surrogate efferent lymph, can be used to provide unique information about spleen responses and substances released to the general circulation during leukemia development.

2023-06-23 | PXD011399 | Pride

The value of Nano Carbon in the lymph node staging of colorectal cancer

Project description:Interventions: experimental group:Preoperative injection of nano - carbon suspension injection;control group:no Primary outcome(s): Number of lymph nodes detected;No. of black stained lymph nodes;Number of positive lymph node;Number of positive black stained lymph nodes;Lymph node staging Study Design: Parallel

| 2686133 | ecrin-mdr-crc

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