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Transcription profiling of human circulating blood reticulocytes from adult and cord blood to provide a robust description of genes transcribed at the final stages of erythroblast maturation

ABSTRACT: RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133 arrays were hybridized with probe from total RNA isolated from blood sampled from 14 umbilical cords and 14 healthy adult humans. Experiment Overall Design: Peripheral blood from 14 normal adult and 14 human umbilical cord were purified to obtain packed red blood cells and their purity was assessed by CELL-DYN4000. The contaminated WBC count was less than 1 out of 1 million cells. Total RNA from purified reticulocytes included in the purified red blood cells was extracted, labeled, and hybridized onto Affymetrix HG-U133 A and B arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Jeffery Lynn Miller

PROVIDER: E-GEOD-6236 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The human reticulocyte transcriptome.

Goh Sung-Ho SH Josleyn Matthew M Lee Y Terry YT Danner Robert L RL Gherman Robert B RB Cam Maggie C MC Miller Jeffery L JL

Physiological genomics 20070403 2

RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133 arrays were hybridized with probe generated from the reticulocyte total RNA (blood obtained from 14 umbilical cords and 14 healthy adult humans). Among the cord and adult reticulocyte profiles, 698 probe sets (488 named genes) were detected in each of the 28 samples. Among the hig ...[more]

PMID: 17405831

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