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Epigenome in brown and white adipose tissue

ABSTRACT: For comparative epigenomic analysis of brown fat and white fat in mice, H3K27ac and PolII ChIP-seq were performed in each depot. H3K27ac histone modification and PolII transcription profiles in mouse brown and white adipose tissues

ORGANISM(S): Mus musculus

SUBMITTER: Hee-Woong Lim

PROVIDER: E-GEOD-63964 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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PRDM16 binds MED1 and controls chromatin architecture to determine a brown fat transcriptional program.

Harms Matthew J MJ Lim Hee-Woong HW Ho Yugong Y Shapira Suzanne N SN Ishibashi Jeff J Rajakumari Sona S Steger David J DJ Lazar Mitchell A MA Won Kyoung-Jae KJ Seale Patrick P

Genes & development 20150201 3

PR (PRD1-BF1-RIZ1 homologous) domain-containing 16 (PRDM16) drives a brown fat differentiation program, but the mechanisms by which PRDM16 activates brown fat-selective genes have been unclear. Through chromatin immunoprecipitation (ChIP) followed by deep sequencing (ChIP-seq) analyses in brown adipose tissue (BAT), we reveal that PRDM16 binding is highly enriched at a broad set of brown fat-selective genes. Importantly, we found that PRDM16 physically binds to MED1, a component of the Mediator ...[more]

PMID: 25644604

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