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Deficiency of multidrug resistance 2 contributes to cell transformation through oxidative stress

ABSTRACT: Mdr2 is the transporter of phosphatidylcholine, which is an essential component of bile. Deficiency of mdr2 causes hepatic inflammation, liver fibrosis and hepatocellular carcinoma. Mdr2-/- MEFs show increased proliferation, spontaneous transformation and tumorigenesis. We used microarray to obtain genome-wide profiling of gene expression underlying the changes in mdr2-/- MEFs. MEFs derived from wt and mdr2-/- littermate embyos were subjected to passage according to 3T3 protocols. RNA was extracted from cells on passage 40 and hybridized on Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Guillaume soubigou

PROVIDER: E-GEOD-72615 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deficiency of multidrug resistance 2 contributes to cell transformation through oxidative stress

Project description:Mdr2 is the transporter of phosphatidylcholine, which is an essential component of bile. Deficiency of mdr2 causes hepatic inflammation, liver fibrosis and hepatocellular carcinoma. Mdr2-/- MEFs show increased proliferation, spontaneous transformation and tumorigenesis. We used microarray to obtain genome-wide profiling of gene expression underlying the changes in mdr2-/- MEFs.

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