Transcription profiling of human endothelial cells or fibroblasts infected with HCMV virus reveals different cell types have different transcriptional responses

ABSTRACT: Human cytomegalovirus infects multiple cell types, including fi-; broblasts and epithelial cells. It penetrates fibroblasts by fusion at; the cell surface but is endocytosed into epithelial cells. In this; report, we demonstrate by electron microscopy that the virus uses; two different routes to enter retinal pigmented epithelial cells,; depending on the cell type in which the infecting virus was; produced. Virus produced in epithelial cells preferentially fuses; with the plasma membrane, whereas fibroblast-derived virus; mostly enters by receptor-mediated endocytosis. Treatment of; epithelial cells with agents that block endosome acidification; inhibited infection by virus produced in fibroblasts but had only a; modest effect on infection by virus from epithelial cells. Epithelial; cell-generated virions had higher intrinsic ‘‘fusion-from-without’’; activity than fibroblast-generated particles, and the two virus; preparations triggered different cellular signaling responses, as; evidenced by markedly different transcriptional profiles. We propose; that the cell type in which a human cytomegalovirus particle; is produced likely influences its subsequent spread and its contribution; to pathogenesis. Experiment Overall Design: A single strain of Human Cytomegalovirus (HCMV), termed BADrUL131, was used to prepare viral stocks from either fibroblast cells or from endothelial cells. These viral preparations (fibroBADrUL131 and epiBADrUL131) were used to infect ARP-19 cells (Human retinal pigmented epithelial cells) for either 6 hours or 10 hours. Total RNA was collected, labeled and used to hybridize to Agilent whole genome oligo microarrays and were normalized to a control reference RNA pool.

ORGANISM(S): Homo sapiens

SUBMITTER: Eain Anthony Murphy 

PROVIDER: E-GEOD-9578 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress