Project description:Gene expression analysis of S. coelicolor M145 wild type strain, and mutants delta0877 and delta 7173. RNA samples were extracted from 48 h culture samples during growth in flasks in defined mineral medium MG supplemented with 5% of yeast extract. Cy5 labelled genomic DNA was used as the common reference.
Project description:Giant cells (GCs)formed by meloidogyne javanica 3 days post infection were isolated by microaspiration from the Arabidopsis Col0 galls. Three independent biological replicates were used for hybridisation. The controls for comparisons were vascular cylinder tissues from uninfected root segments at equivalent positions to the galls in the infected roots. RNA extracted directly from either the controls or the GCs were amplified two times with a RNA polimerase linear amplification based approach
Project description:Transcriptional profiles from Acidithiobacillus ferrooxidans type strain ATCC23270 grown in the presence of iron or elemental sulfur as energy source until mid logarithmic phase were compared. Cells were harvested after 48 hs of growth in 9K-FeII pH 1.6 and after 120 hs of growth in 9K-S0 pH 3.5. Pellets were washed to remove precipitates, frozen and stored at -80C until RNA extraction.<br><br>In toto 8 different hybridizations were performed using 4 independent biological samples of A. ferrooxidans (with dye-swap), 4 arrays of array design FCV-CNRS AFE Oligoarray v.1 and 4 arrays of array design FCV-CNRS AFE Oligoarray v.2. This experimental design produced 8 raw data files and 1 transformed and/or normalized data file.
Project description:Cross-species hybridizations of Sordaria macrospora targets on Neurospora crassa microarrays were performed with targets derived from the S. macrospora wild type undergoing sexual development (wt.sex) and the mutant strains dSmtA-1 and dSmtA-2. For each strain, two independent experiments were carried out with a dye switch in the second experiment.<br><br>The following slide/target/dye combinations were done:<br><br>slide N16-86: Cy3-target: wt.sex (1), Cy5-target: dSmtA-1 (1)<br><br>slide N16-87: Cy3-target: dSmtA-1 (2), Cy5-target: dSmtA-2(2)<br><br>slide N16-88: Cy3-target: dSmtA-2 (1), Cy5-target: wt.sex (2)
Project description:E. coli MG1655 was grown in MOPS minimal glucose medium at 37 degrees C with aeration to an OD600 of 0.4 - 0.5, and HOCl was added to a final concentration of 400 ?M. 0.5 ml samples were collected in liquid nitrogen immediately before, 5 min after, and 10 min after HOCl addition, and total RNA was prepared using the RNeasy? Midi kit (Qiagen). cDNA synthesis, array hybridization to Affymetrix GeneChip E. coli genome 2.0 Arrays, and imaging were performed according to Affymetrix guidelines at the Affymetrix and Microarray Core facility at the University of Michigan, Ann Arbor.
Project description:Expression profile of meiosis in strain with Ndt80 inducible by addition of Estradiol (as in Carlile and Amon 2008), in three time points from addition of Estradiol.
Project description:B. cenocepacia J2315 was grown to mid-stationary phase in basal salt medium with two different substrates: 20 mM glucose or 40 mM glycerol. Cells were harvested after 30 hours incubation at 37 degrees centigrade. <br>The expression profile was compared to cells grown on the same medium and harvested in mid-log phase.
Project description:Role of transcription factor Tye7p in condition of biofilm (at 24, 32 and 40h growth) and planktonic culture for the yeast Candida albicans
Project description:Cabernet Sauvignon in vitro plantlets were experimentally infected with the E. lata strain NE85-1 and compared to healthy controls.