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Chromatin immunoprecipitation of Drosophila DNA to identify nucleosomes bound by RNA polymerase II

ABSTRACT: Crosslinked chromatin was digested with Micrococcal nuclease and immunopurified for Rpb3, a subunit of RNA Polyermase II. chIP'd DNA was hybridized to Drosophila tiling arrays and nucleosomes that were bound by Pol II were mapped.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Cizhong Jiang

PROVIDER: E-MEXP-1515 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nucleosome organization in the Drosophila genome.

Mavrich Travis N TN Jiang Cizhong C Ioshikhes Ilya P IP Li Xiaoyong X Venters Bryan J BJ Zanton Sara J SJ Tomsho Lynn P LP Qi Ji J Glaser Robert L RL Schuster Stephan C SC Gilmour David S DS Albert Istvan I Pugh B Franklin BF

Nature 20080413 7193

Comparative genomics of nucleosome positions provides a powerful means for understanding how the organization of chromatin and the transcription machinery co-evolve. Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from the yeast Saccharomyces cerevisiae. Like Saccharomyces, Drosophila nucleosomes are organized around active transcription start sites in a canonical -1, nucleosome-fre ...[more]

PMID: 18408708

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