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Mapping of mononucleosomal DNA in wild type Drosophila

ABSTRACT: Crosslinked chromatin from wild-type flies was digested with micrococcal nuclease. Mononucleosomal DNA was gel-purified and hybridized to Affymetrix tiling arrays.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Cizhong Jiang

PROVIDER: E-MEXP-1519 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nucleosome organization in the Drosophila genome.

Mavrich Travis N TN Jiang Cizhong C Ioshikhes Ilya P IP Li Xiaoyong X Venters Bryan J BJ Zanton Sara J SJ Tomsho Lynn P LP Qi Ji J Glaser Robert L RL Schuster Stephan C SC Gilmour David S DS Albert Istvan I Pugh B Franklin BF

Nature 20080413 7193

Comparative genomics of nucleosome positions provides a powerful means for understanding how the organization of chromatin and the transcription machinery co-evolve. Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from the yeast Saccharomyces cerevisiae. Like Saccharomyces, Drosophila nucleosomes are organized around active transcription start sites in a canonical -1, nucleosome-fre ...[more]

PMID: 18408708

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