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Chromatin immunoprecipitation of yeast transcription machinary using oligonucleotide arrays

ABSTRACT: A canonical organization of the transcription pre-initiation complex and its regulators. High-resolution genome-wide mapping of the yeast transcription machinery was performed for each factor using ChIP-chip.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Bryan Venters

PROVIDER: E-MEXP-1677 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A canonical promoter organization of the transcription machinery and its regulators in the Saccharomyces genome.

Venters Bryan J BJ Pugh B Franklin BF

Genome research 20090105 3

The predominant organizational theme by which the transcription machinery and chromatin regulators are positioned within promoter regions or throughout genes in a genome is largely unknown. We mapped the genomic location of diverse representative components of the gene regulatory machinery in Saccharomyces cerevisiae to an experimental resolution of <40 bp. Sequence-specific gene regulators, chromatin regulators, mediator, and RNA polymerase (Pol) II were found primarily near the downstream bord ...[more]

PMID: 19124666

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