Project description:Transcriptional profiling of preangiogenic and angiogenic tumor nodules was conducted to identify genes that were differentially expressed during the transition from the preangiogenic to the angiogenic phenotype.
Project description:The transcriptome of early local kidney inflammation was profiled. Rat kidney tubules were injected with uropathogenic E. coli or PBS control. After incubation time, kidney samples were processed and transcription was assayed. Three rats were used per condition.
Project description:In order to elucidate the molecular mechanisms of action of Phenytoin, we examined by microarrays the effects of prolonged administration of Phenytoin on gene expression in hippocampus and frontal cortex of Sprague-Dawley rats chronically treated with Phenytoin.
Project description:Many epithelia have contact with air-liquid interfaces. This applies particularly to the lung, where one of the epithelial cell types (the surfactant secreting alveolar type II [AT II] cells) even project into the air. This specific environment may be of considerable physiological relevance; however, only few data exist to provide a satisfying description. This is mainly due to the difficulty to manipulate cell-air contacts in a specific way. In previous investigations, using new microscopic approaches, we found that the presence of an air-liquid interface leads to a paradoxical situation: it is a potential threat that may cause cell injury, but also a potent stimulus for the cells: AT II cells respond promptly, and show sustained Ca2+-signals that activate exocytosis. In this report, gene chip analysis was performed to test the concept of an Air-liquid interface IAL as a mechanical stimulus, stress factor and/or regulator of the AT II cells. For this purpose, cultured AT II cells were exposed to two different interfacial conditions (inverted and conventional interface). The results support the concept that the IAL is a strong stimulus for AT II cells inducing cellular responses that seem to be closely associated with mechanotransduction, stress response, and alveolar repair.
Project description:This microarray study compared the gene expression profile of rat tail tendon tissue in three different developmental stages: embryonic day 21, postnatal 3 weeks and postnatal 6 weeks.<br><br><br><br>Key words: rat tail tendon, tissue development, embryonic and postnatal
Project description:Using pangenomic cDNA microarrays and qPCR techniques, we identified the genes regulated by calcitriol (1,25 (OH)D3, 10 nM) in dorsal root ganglia and/or Schwann cells. After 24 hours of calcitriol supplementation, we found a modified expression of many genes involved in axogenesis and myelination.
Project description:Mid-shaft fracture stimulates bone lengthening by increasing linear growth at the growthplate. This project studied changes in mRNA in the proximal growthplate after a mid-shaft fracture in a rat model. Experiment Overall Design: Study of rat proximal femoral growthplate after mid-shaft fracture in 4 week old Sprague-Dawley female rats. RNA from two rats were pooled for each sample. Proximal femoral growthplate was studied from the fractured femora and the intact contralateral femora.