Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

42 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of neonatal rat ventricular cardiac myocytes treated with interleukin 33 for 0.5, 1 or 2 hours

ABSTRACT: Study of the effect of interleukin 33 (IL33) on transcript expression in neonatal rat ventricular cardiac myocytes at 0.5, 1 or 2 h

ORGANISM(S): Rattus norvegicus

SUBMITTER: Angela Clerk

PROVIDER: E-MEXP-1912 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

The early transcriptomic response to interleukin 1β and interleukin 33 in rat neonatal cardiomyocytes.

Barrett Oliver P T OP Yndestad Arne A Marshall Andrew K AK Sugden Peter H PH Clerk Angela A

Cytokine 20121207 2

In the heart, inflammatory cytokines including interleukin (IL) 1β are implicated in regulating adaptive and maladaptive changes, whereas IL33 negatively regulates cardiomyocyte hypertrophy and promotes cardioprotection. These agonists signal through a common co-receptor but, in cardiomyocytes, IL1β more potently activates mitogen-activated protein kinases and NFκB, pathways that regulate gene expression. We compared the effects of external application of IL1β and IL33 on the cardiomyocyte trans ...[more]

PMID: 23219998

Similar Datasets

Transcription profiling by array of rat ventricular cardiac myocytes treated with interleukin 1 beta

Project description:Study of the effects of interleukin 1 beta on transcript expression in ventricular cardiac myocytes

2010-04-01 | E-MEXP-1960 | biostudies-arrayexpress

Effect of 30 or 90 min exposure to endothelin-1 on the cardiac myocyte transcriptome

Project description:Cardiac myocytes were exposed to 100 nM endothelin-1 for 30 or 90 min to determine effects on mRNA expression using Exon arrays

2012-10-01 | E-MEXP-3393 | biostudies-arrayexpress

Effect of 60 min exposure to endothelin-1 on the cardiac myocyte transcriptome

Project description:Cardiac myocytes were unstimulated or exposed to 100 nM endothelin-1 for 60 min

2012-10-01 | E-MEXP-3394 | biostudies-arrayexpress

Transcription profiling by array of Rattus norvegicus cardiomyocytes to investigate the effects of 30 or 60 min phenylephrine exposure on gene expression

Project description:Rat neonatal cardiomyocytes were exposed to 100 uM phenylephrine for 0, 30 or 60 mins

2013-01-16 | E-MEXP-3678 | biostudies-arrayexpress

Transcriptional profiling by array to study the effect of adenoviruses encoding the FLAG epitope or full-length antisense sequence to Atf3 on transcriptional regulation in rat cardiac myocytes

Project description:Cardiomyocytes, the terminally-differentiated contractile cells of the heart, undergo hypertrophic growth in response to endothelin-1. This is associated with changes in mRNA expression of immediate early genes (IEGs). Atf3 is a member of the ATF/CREB family transcription factors which bind as dimers to the cAMP response element (CRE). Atf3 mRNA is particularly associated with cell stress responses and is implicated in negative feedback regulation of gene expression. We confirmed that endothelin-1 promotes expression of Atf3 mRNA and protein in cardiomyocytes and that it is an IEG. Atf3 expression was transient and maximal at 0.5-1 h. We used adenoviruses for expression of antisense Atf3 RNA in cardiomyocytes to inhibit upregulation of Atf3 protein in response to endothelin-1. The effects on the cardiomyocyte transcriptome in control cells and cells exposed to endothelin-1 were examined using Affymetrix rat exon 1.0 ST arrays with data analysis using GeneSpring GX11.5. We identified transcripts that were upregulated by endothelin-1 for which antisense Atf3 significantly (FDR<0.05) enhanced or inhibited expression, respectively. The basal levels of other transcripts that are upregulated by endothelin-1 were also increased by antisense Atf3, though antisense Atf3 had no further effect on the response to endothelin-1. The data were validated by quantitative PCR of selected IEG mRNAs examining the effects at different times.

2012-10-01 | E-MEXP-3392 | biostudies-arrayexpress

Transcriptional profiling of rat heart ventricular myocytes treated either with PD184352 alone or PD184352 and endothelin-1.

Project description:Effects of PD184352 on RNA profiles in neonatal rat cardiomyocytes exposed to endothelin-1

2010-05-26 | E-MEXP-2516 | biostudies-arrayexpress

Transcription profiling of rat neonatal ventricular cardiac mycocytes exposed to endothelin-1 and C3 toxin

Project description:Effects of C3 toxin on RNA profiles in neonatal rat ventricular cardiac myocytes exposed to endothelin-1 (1 h)

2010-05-25 | E-MEXP-1977 | biostudies-arrayexpress

Transcription profiling by array of Rattus norvegicus cardiomyocytes to investigate the effects of PD184352 and BI-D1870 on changes in expression induced by endothelin-1

Project description:Cardiomyocytes were exposed to PD184352, BI-D1870 or endothelin-1 alone, or to endothelin-1 in the presence of each inhibitor

2013-01-16 | E-MEXP-3679 | biostudies-arrayexpress

Transcription and translation profiling of rat cardiomyocytes treated with insulin

Project description:Effect of insulin on the cardiomyocyte global transcriptome and RNAs recruited to polysomes. For comparison with endothelin-1

2010-07-04 | E-MEXP-2527 | biostudies-arrayexpress

Transcription profiling of H2O2 treated rat cardiac myocytes

Project description:Title: Changes in gene expression affected by H2O2 in cardiac myocytes. Description: We aim to identify the changes in gene expression in response to oxidative stress in rat neonatal ventricular myocytes. Oxidative stress will be induced by dosing neonatal ventricular myocyte cultures with 0.2, 0.1 and 0.04mM hydrogen peroxide at 2, 4 and 8 hr time points using unstimulated myocytes as control.

2011-11-08 | E-MIMR-12 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data