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Transcription profiling, Rpd3 ChIP-chip and histone H4 and TATA binding protein ChIP-chip of wild type, cti6 and sfl1 yeast mutants

ABSTRACT: Effects of genetic modification of trans-acting transcription factors and a chromatin remodeler on noncoding RNA transcription and trans-acting factor binding at loci in yeast.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Robin Dowell

PROVIDER: E-MEXP-2269 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Toggle involving cis-interfering noncoding RNAs controls variegated gene expression in yeast.

Bumgarner Stacie L SL Dowell Robin D RD Grisafi Paula P Gifford David K DK Fink Gerald R GR

Proceedings of the National Academy of Sciences of the United States of America 20090930 43

The identification of specific functional roles for the numerous long noncoding (nc)RNAs found in eukaryotic transcriptomes is currently a matter of intense study amid speculation that these ncRNAs have key regulatory roles. We have identified a pair of cis-interfering ncRNAs in yeast that contribute to the control of variegated gene expression at the FLO11 locus by implementing a regulatory circuit that toggles between two stable states. These capped, polyadenylated ncRNAs are transcribed acros ...[more]

PMID: 19805129

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