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Low dose PDT - Murine Cells

ABSTRACT: Murine prostate carcinoma cell lines TRAMP-C1 and TRAMP-C2 were grown for 16 h at 37M-0C with photosensitizer 5-aminolaevulinic acid (5-ALA; 50 M-5g/ml). Then, the cells were sublethally irradiated with laser light (635 nm) at room temperature (using RPMI1640 without phenol red as medium). After photodynamic therapy, the cells were cultivated at 37M-0C for 4 h and 24 h, respectively. After that time total RNA was extracted for expression profiling.

ORGANISM(S): Mus musculus

SUBMITTER: Alexander Buchner

PROVIDER: E-MEXP-3016 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Induction of immune mediators in glioma and prostate cancer cells by non-lethal photodynamic therapy.

Kammerer Robert R Buchner Alexander A Palluch Patrick P Pongratz Thomas T Oboukhovskij Konstantin K Beyer Wolfgang W Johansson Ann A Stepp Herbert H Baumgartner Reinhold R Zimmermann Wolfgang W

PloS one 20110630 6

<h4>Background</h4>Photodynamic therapy (PDT) uses the combination of photosensitizing drugs and harmless light to cause selective damage to tumor cells. PDT is therefore an option for focal therapy of localized disease or for otherwise unresectable tumors. In addition, there is increasing evidence that PDT can induce systemic anti-tumor immunity, supporting control of tumor cells, which were not eliminated by the primary treatment. However, the effect of non-lethal PDT on the behavior and malig ...[more]

PMID: 21738796

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