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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Ire1 double mutant vs wild-type in response to tunicamycin

ABSTRACT: Arabidopsis thaliana wild-type and ire1a/ire1b double mutant plants were treated with tunicamycin. RNA was extracted and subjected to microarray analysis.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yuji Iwata

PROVIDER: E-MEXP-3186 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor.

Nagashima Yukihiro Y Mishiba Kei-Ichiro K Suzuki Eiji E Shimada Yukihisa Y Iwata Yuji Y Koizumi Nozomu N

Scientific reports 20110701

IRE1 plays an essential role in the endoplasmic reticulum (ER) stress response in yeast and mammals. We found that a double mutant of Arabidopsis IRE1A and IRE1B (ire1a/ire1b) is more sensitive to the ER stress inducer tunicamycin than the wild-type. Transcriptome analysis revealed that genes whose induction was reduced in ire1a/ire1b largely overlapped those in the bzip60 mutant. We observed that the active form of bZIP60 protein detected in the wild-type was missing in ire1a/ire1b. We further ...[more]

PMID: 22355548

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