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Chromatin immunoprecipitation to identify H-NS and RNA polymerase (beta-subunit) binding sites on the Salmonella typhimurium chromosome

ABSTRACT: Chromatin immunoprecipitation experiment to identify H-NS and RNA polymerase (beta-subunit) binding sites on the Salmonella Typhimurium chromosome

INSTRUMENT(S): other

ORGANISM(S): Salmonella typhimurium

SUBMITTER: Douglas Hurd

PROVIDER: E-MEXP-806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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H-NS mediates the silencing of laterally acquired genes in bacteria.

Lucchini Sacha S Rowley Gary G Goldberg Martin D MD Hurd Douglas D Harrison Marcus M Hinton Jay C D JC

PLoS pathogens 20060801 8

Histone-like nucleoid structuring protein (H-NS) is a modular protein that is associated with the bacterial nucleoid. We used chromatin immunoprecipitation to determine the binding sites of H-NS and RNA polymerase on the Salmonella enterica serovar Typhimurium chromosome. We found that H-NS does not bind to actively transcribed genes and does not co-localize with RNA polymerase. This shows that H-NS principally silences gene expression by restricting the access of RNA polymerase to the DNA. H-NS ...[more]

PMID: 16933988

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