Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling time series of Bacillus cereus from cultures grown in media with bile salts


ABSTRACT: RNA was isolated from control and bile salt cultures 15, 30, and 60 minutes after shift (named C15,C30,C60 and BC15,BS30,BS60, repectively) and hybridized against the reference RNA (C0) isolated immediatly after the shift.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Bacillus cereus

SUBMITTER: Simen Kristoffersen 

PROVIDER: E-MEXP-896 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Low concentrations of bile salts induce stress responses and reduce motility in Bacillus cereus ATCC 14579 [corrected].

Kristoffersen Simen M SM   Ravnum Solveig S   Tourasse Nicolas J NJ   Økstad Ole Andreas OA   Kolstø Anne-Brit AB   Davies William W  

Journal of bacteriology 20070511 14


Tolerance to bile salts was investigated in forty Bacillus cereus strains, including 17 environmental isolates, 11 dairy isolates, 3 isolates from food poisoning outbreaks, and 9 other clinical isolates. Growth of all strains was observed at low bile salt concentrations, but no growth was observed on LB agar plates containing more than 0.005% bile salts. Preincubation of the B. cereus type strain, ATCC 14579, in low levels of bile salts did not increase tolerance levels. B. cereus ATCC 14579 was  ...[more]

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