Total RNA sequencing for Mus musculus bone marrow lymphoid committed cell populations
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ABSTRACT: MPP2, MPP3 and CLP populations were isolated by fluorescence-activated cell sorting (FACS) from the BM of B6 mice based on the surface expression of VCAM1, FLT3 and IL7Ra within LSK population. On average 100K cells were analyzed for each population in 3 independent biological experiments. We generated sequence libraries from these low RNA inputs using the SMARTer Stranded RNA-Seq and sequenced at high depth of coverage to generate ~2x120 M reads per biological replicate.
INSTRUMENT(S): Illumina HiSeq 2000
ORGANISM(S): Mus musculus
SUBMITTER: Smahane Chalabi
PROVIDER: E-MTAB-11217 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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