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CD27 signaling in CD8+ T cells in vivo

ABSTRACT: Wild type or Cd27-/- mice of 8-12 weeks old were immunized intranasally with 500 ug ovalbumin (OVA) protein and 1 ug cholera toxin in 50 ul HBSS. Spleen, draining lymph node (DLN) or lung were isolated at day 3, day 4 or day 8 after immunization. Single cell suspensions of indicated organs were prepared and OT-I T cells were isolated from this using flow cytometric cell sorting.

ORGANISM(S): Mus musculus

SUBMITTER: Iris de Rink

PROVIDER: E-MTAB-1772 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CD8+ T cells produce the chemokine CXCL10 in response to CD27/CD70 costimulation to promote generation of the CD8+ effector T cell pool.

Peperzak Victor V Veraar Elise A M EA Xiao Yanling Y Babala Nikolina N Thiadens Klaske K Brugmans Marieke M Borst Jannie J

Journal of immunology (Baltimore, Md. : 1950) 20130812 6

Various cell types can produce the chemokine CXCL10 in response to IFN-γ stimulation. CXCL10 is generally viewed as a proinflammatory chemokine that promotes recruitment of CD8÷ and Th1-type CD4÷ effector T cells to infected or inflamed nonlymphoid tissues. We show that CXCL10 plays a role during CD8÷ T cell priming in the mouse. Genome-wide expression profiling revealed the Cxcl10 gene as a target of CD27/CD70 costimulation in newly activated CD8÷ T cells. CD27/CD70 costimulation is known to pr ...[more]

PMID: 23940275

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