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Genome-wide expression analysis in normal and immortalised and transformed human cell lines

ABSTRACT: Analysis of expression changes in the fetal human lung fibroblast cell line MRC5 over time (two time points: 50 and 100 population doublings) during immortalisation with the catalytic component of telomerase (hTERT) and in vitro transformation with SV40 Large T antigen and oncogenic H-RAS

ORGANISM(S): Homo sapiens

SUBMITTER: Jose de las Heras

PROVIDER: E-MTAB-2003 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Immortality, but not oncogenic transformation, of primary human cells leads to epigenetic reprogramming of DNA methylation and gene expression.

Gordon Katrina K Clouaire Thomas T Bao Xun X XX Kemp Sadie E SE Xenophontos Maria M de Las Heras Jose Ignacio JI Stancheva Irina I

Nucleic acids research 20131226 6

Tumourigenic transformation of normal cells into cancer typically involves several steps resulting in acquisition of unlimited growth potential, evasion of apoptosis and non-responsiveness to growth inhibitory signals. Both genetic and epigenetic changes can contribute to cancer development and progression. Given the vast genetic heterogeneity of human cancers and difficulty to monitor cancer-initiating events in vivo, the precise relationship between acquisition of genetic mutations and the tem ...[more]

PMID: 24371281

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Genome-wide DNA methylation analysis of promoters in normal and immortalised and transformed human cell lines

Project description:Analysis of DNA methylation changes at promoters in the fetal human lung fibroblast cell line MRC5 over time (two time points: 50 and 100 population doublings) during immortalisation with the catalytic component of telomerase (hTERT) and in vitro transformation with SV40 Large T antigen and oncogenic H-RAS

2013-11-11 | E-MTAB-2005 | biostudies-arrayexpress

Transcription profiling of human umbilical vein endothelial cells after transfection with the anti-apoptotic protein vFLIP/K13 of human herpesvirus type-8 (HHV-8)

Project description:Modulation of the transcriptome of human umbilical vein endothelial cells (HUVEC) by the anti-apoptotic protein vFLIP/K13 of human herpesvirus type-8 (HHV-8)

2008-11-14 | E-MEXP-1469 | biostudies-arrayexpress

IMR90 hTERT

Project description:Transcription profiling from young and pre-senescent IMR90 cells, transfected either with hTERT (pBabe-puro-hTERT) or vector control (pBabe-puro). Population doubling values are contained in Characteristics[generation] column.

2014-06-17 | E-MTAB-2087 | biostudies-arrayexpress

High-throughput sequencing of AGO-immunoprecipitating miRs in human senescent fibroblast WI-38

Project description:In humans, there are four Ago proteins (Ago1M-bM-^@M-^S4) and AGO1- and 2 were previously implicated in TGS induced by exogenous siRNAs and microRNAs (miRs) directed against gene promoter transcripts via promotion of changes in histone covalent modifications and DNA methylation. Not-with-standing, many mechanistic details of this process remain poorly defined in human cells, and very little is known about the identity of possible endogenous signals, which may drive this process in human cells. Given the evolutionary conserved role of siRNAs and AGO proteins in TGS and heterochromatin formation, we set out to analyse their possible involvement in senesence-associated repression of E2F target genes. To obtain a detailed picture of AGO-immunoprecipitating miRs (RIP) in senescent cells, we used next-generation sequencing (NGS)(RIP-Seq). We also included histone H3 dimethylated on lysine 9 (H3K9me2) in this analysis to assign potential AGO2-interacting miRs to a repressive chromatin state and unfractionated, cellular RNA from senescent cells for normalisation. Determination of AGO AGO-immunoprecipitating miRs in WI-38 senescent human fibroblast

2011-12-17 | E-GEOD-34494 | biostudies-arrayexpress

Transcription profiling of primary mouse keratinocytes infected with Myc, MycV394D or control virus supernatants and treated with or without TGF beta 1 for 12 hours

Project description:Primary murine keratinocytes were infected with Myc, MycV394D or control virus supernatants and treated with or without TGF beta 1 for 12 hours. MycV394D is a Myc mutant, which does not bind Miz-1 anymore but is still able to bind to E-box sequences (Mol Cell 10:509-521, 2002).

2005-12-20 | E-MEXP-497 | biostudies-arrayexpress

Transcription profiling of human mesenchymal stem cells transformed with lentiviral vectors of TERT, E6, SV40 and RAS

Project description:Mesenchymal Stem Cells are transformed by step-wise stable transfection with lentiviral vectors of TERT, E6, E7, SV40, and Ras.

2007-04-01 | E-MEXP-563 | biostudies-arrayexpress

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STATs Shape the Active Enhancer Landscape of T Cell Populations

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2012-11-24 | E-GEOD-40463 | biostudies-arrayexpress

A novel unusual chromosome 11 abnormality: a homozygous somatic deletion of the entire WT1 gene within a heterozygous 11p13 deletion and UPD limited to 11p15 in a Wilms tumor and establishment of an immortalized cell line

Project description:We describe a stromal predominant Wilms tumor with a complex, tumor specific chromosome 11 aberration: a homozygous deletion of the entire WT1 gene within a heterozygous 11p13 deletion and an additional region of uniparental disomy (UPD) limited to 11p15.5-p15.2 including the IGF2 gene. The tumor carried a heterozygous p.T41A mutation in CTNNB1. Cells established from the tumor carried the same chromosome 11 aberration, but a different, homozygous p.S45Î CTNNB1 mutation. Uniparental disomy (UPD) 3p21.3pter lead to the homozygous CTNNB1 mutation. The tumor cell line was immortalized with telomerase (TERT) and a novel triple ts mutant SV40 large T antigen (LT). This cell line is cytogenetically stable and can be grown indefinitely. It is a valuable tool to study the effect of a complete lack of WT1 in tumor cells. The tumor cell line and the immortalized cells have a limited potential for muscle/osteogenic/adipogenic differentiation similar to all other WT1 mutant cell lines. Array expression experiment of Wilms tumor cell lines

2016-06-01 | E-GEOD-71265 | biostudies-arrayexpress

Id2 and E proteins orchestrate the initiation and maintenance of MLL-rearranged acute myeloid leukemia

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-06-29 | E-GEOD-68463 | biostudies-arrayexpress