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Separated transcriptome analysis of microglial cells and oligodendrocytes in the brain of P4 rats, in intra-uterine growth restriction or control conditions

ABSTRACT: Females were exposed during pregnancy to a normal or low protein diet (as described in Buffat et al, J. Pathol, 2007 and Zana-Taieb et al, J. Pathol, 2015). Brains cells were dissociated using the Neural Tissue Dissociation Kit of Miltenyi Biotec,in the presence of papain. Then Magnetic Bead coupled antibody (MACS) was used to sort the cells at P4. Microglial cells were sorted using the surface marker CD11b, and the oligodendrocyte cells using the O4 marker.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Daniel Vaiman

PROVIDER: E-MTAB-4647 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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