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Effects of low protein diet-induced Intra-Uterine Growth Restriction in Rat microglial brain cells at P0 and P4. Double Hit at P4 with subclinical doses of IL1B

ABSTRACT: Female rats were exposed during pregnancy to a normal or low protein diet (as described in Bufffat et al, J. Pathol, 2007 and Zana-Taieb et al, J. Pathol, 2015; Rideau Batista Novais, Glia, 2016), then injected with low doses of interleukin1B (20µg/kg) at P1, for the P4 samples. LPD effects were also addressed at birth (P0) Brains cells were dissociated using the Neural Tissue Dissociation Kit of Miltenyi Biotec,in the presence of papain. Then Magnetic Bead coupled antibody (MACS) was used to sort the cells at P4 or at P0. Microglial cells were sorted using the surface marker CD11b. Transcriptome analysis was carried out on this subset of cells, using triplicates of a pool of three different mRNA samples from each experimental group.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Daniel VAIMAN

PROVIDER: E-MTAB-6630 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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