Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

35 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array to investigate the long term effects of preeclampsia in hearts using a mouse model

ABSTRACT: Long-term consequences of preeclampsia were studied by high-throughput approaches (Transcriptomics, Luminex cytokine profile) in mice 8 months after the disease in the heart, in the endothelial cells and in the plasma. In the heart we found a persisting ventricular hypertrophy with an altered histology and an abnormal ultrasound Doppler profile under effort simulation by dobutamine injection. The transcriptomic profile of the endothelium revealed a deregulation for 1149 genes (327 down-regulated and 822 up-regulated, with a threshold of 1.5, p<0.05). The up-regulated genes could be grouped consistently in gene pathways and gene ontology terms mainly focused on Inflammation and Stress sensu lato. A cytokine profile of the mouse serum was carried out. Using a combination of 8 cytokines (Cxcl13, Cxcl16, Cxcl11, Il-16, Il-10, Il-2, Il-4 and Ccl1) in a Discriminant Analysis, we were able to separate unambiguously the mice having had a preeclamptic pregnancy from the controls. Seven out of eight of these cytokines (with the exception of Il-16) varied in the same direction in the endothelium and in the plasma. In sum, our study shows that preeclampsia alone is able to trigger considerable long-term consequences, and suggests that specific cytokines could help to improve the follow-up of patients long after a preeclamptic pregnancy.

ORGANISM(S): Mus musculus

SUBMITTER: Daniel VAIMAN

PROVIDER: E-MTAB-7357 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Endothelial effects of preeclampsia in the long-term using the STOX1 model

Project description:Long-term consequences of preeclampsia were studied by high-throughput approaches (Transcriptomics, Luminex cytokine profile) in mice 8 months after the disease in the heart, in the endothelial cells and in the plasma. In the heart we found a persisting ventricular hypertrophy with an altered histology and an abnormal ultrasound Doppler profile under effort simulation by dobutamine injection. The transcriptomic profile of the endothelium revealed a deregulation for 1149 genes (327 down-regulated and 822 up-regulated, with a threshold of 1.5, p<0.05). The up-regulated genes could be grouped consistently in gene pathways and gene ontology terms mainly focused on Inflammation and Stress sensu lato. A cytokine profile of the mouse serum was carried out. Using a combination of 8 cytokines (Cxcl13, Cxcl16, Cxcl11, Il-16, Il-10, Il-2, Il-4 and Ccl1) in a Discriminant Analysis, we were able to separate unambiguously the mice having had a preeclamptic pregnancy from the controls. Seven out of eight of these cytokines (with the exception of Il-16) varied in the same direction in the endothelium and in the plasma. In sum, our study shows that preeclampsia alone is able to trigger considerable long-term consequences, and suggests that specific cytokines could help to improve the follow-up of patients long after a preeclamptic pregnancy.

2019-06-01 | E-MTAB-7358 | biostudies-arrayexpress

Placental Effects of Aspirin treatment in preeclamptic women

Project description:Aspirin is currently the only drug used in preeclampsia, a major hypertensive disorder of pregnancy. The counselled doses are low (75-150 mg/day), and the actual effects of aspirin are not well understood, especially in the target organ, the placenta.This is the aim of the present study.

2022-12-31 | E-MTAB-12434 | biostudies-arrayexpress

Curing Preeclampsia induced by STOX1A expression in Mouse Placentas by Dietary Tetrahydrobiopterin Treatment

Project description:STOX1A overexpression in mice placenta triggers a preeclamptic phenotype in the mothers, with hypertension, proteinuria and kidney alterations (Doridot et al, Hypertension, 2013), this being connected with NO depletion and increase of nutrosative stress (Doridot et al, Antiox Redox Signal, 2014). Tetrahydrobiopterin (BH4) is a cofactor essential for NO production. We treated preeclamptic mice with this drug, and analyzed placental gene expression in four groups (Control mice, Control mice with BH4, Preeclamptic mice and preeclamptic mice with BH4).

2021-05-01 | E-MTAB-9314 | biostudies-arrayexpress

Transcriptomic effects of human STOX1B overexpression on the mouse placenta

Project description:STOX1B, the short protein-coding isoform of STOX1 (227 amino acids), has been shown to present opposite effects than the one of STOX1B in trophoblast cell models. Here, we investigate the overexpression of STOX1B in vivo in the mouse placenta. The changes accompany maternal effects on blood pressure and proteinuria reminiscent of preeclampsia, and effects of Intra Uterine Growth Restriction upon the foetuses.

2024-10-31 | E-MTAB-14457 | biostudies-arrayexpress

Comparison of BeWo cells expressing WT or 3 variants/mutation of the STOX1 transcription factor

Project description:Three variants of STOX1 (Y153H, T188N, and R364X) were associated to diseases of pregnancy (preeclampsia and HELLP syndrome). Here we analyze the expression profile of these mutations (6 independent clones) starting from two independent Knocked-Out cell lines (KO5 and KO21).

2023-12-31 | E-MTAB-13357 | biostudies-arrayexpress

Separated transcriptome analysis of microglial cells and oligodendrocytes in the brain of P4 rats, in intra-uterine growth restriction or control conditions

Project description:Females were exposed during pregnancy to a normal or low protein diet (as described in Buffat et al, J. Pathol, 2007 and Zana-Taieb et al, J. Pathol, 2015). Brains cells were dissociated using the Neural Tissue Dissociation Kit of Miltenyi Biotec,in the presence of papain. Then Magnetic Bead coupled antibody (MACS) was used to sort the cells at P4. Microglial cells were sorted using the surface marker CD11b, and the oligodendrocyte cells using the O4 marker.

2016-10-14 | E-MTAB-4647 | biostudies-arrayexpress

Expression of brain gene at P4 and P10 post-coÃ¯tus in the rat, after protein restriction of the mother regimen during gestation

Project description:Total brain was collected from newborn rats that were growth-restricted obtained by feeding the females a low-protein diet during pregnancy. The model was extensively described in Buffat et al, J. Pathology 2007, and Zana-Taieb et al, J. Pathology, 2015.

2016-10-14 | E-MTAB-4646 | biostudies-arrayexpress

Effects of low protein diet-induced Intra-Uterine Growth Restriction in Rat Whole brain at 28 days, and on microglial brain cells at P4. Double Hit with subclinical doses of IL1B, Rescue by Carbetocin treatment.

Project description:Female rats were exposed during pregnancy to a normal or low protein diet (as described in Bufffat et al, J. Pathol, 2007 and Zana-Taieb et al, J. Pathol, 2015; Rideau Batista Novais, Glia, 2016), then injected with low doses of interleukin1B (20µg/kg) at P1, for the P4 samples. Brain samples were taken in Trizol at 28 days post-natal for a subset of the animals For the P4 samples, brains cells were dissociated using the Neural Tissue Dissociation Kit of Miltenyi Biotec,in the presence of papain. Then Magnetic Bead coupled antibody (MACS) was used to sort the cells. Microglial cells were sorted using the surface marker CD11b. Transcriptome analysis was carried out on these samples using triplicates of a pool of three different mRNA samples from each experimental group.

2018-12-30 | E-MTAB-6631 | biostudies-arrayexpress

CD74 downregulation in placental macrophages and its influence on trophoblast cells.

Project description:Preeclampsia is a disease of pregnant women, which is characterized by hypertension, proteinuria and chronic inflammation. There is a growing body of evidence that cause of preeclampsia lies within immunological aspect of pregnancy. This study aimed to analyze the role of CD74 in preeclampsia with a focus on its influence on communication between placental macrophages (Hofbauer cells) and trophoblasts. We have found CD74 to be highly dysregulated in preeclamptic placenta by real-time RT-PCR and Western blot methods. We identified Hofbauer cells to express the highest levels of CD74 in placenta by immunofluorescence and flow cytometry and that CD74 in preeclamptic Hofbauer cells is lower than in controls. We have performed a transcriptome analysis on human blood monocyte-derived macrophages that were non- or IL-4-activated and treated with small interfering RNA against CD74 (siRNA CD74) or non-targeting siRNA (siRNA non-targeting) as control.

2015-04-29 | E-MTAB-3309 | biostudies-arrayexpress

Effects of low protein diet-induced Intra-Uterine Growth Restriction in Rat microglial brain cells at P0 and P4. Double Hit at P4 with subclinical doses of IL1B

Project description:Female rats were exposed during pregnancy to a normal or low protein diet (as described in Bufffat et al, J. Pathol, 2007 and Zana-Taieb et al, J. Pathol, 2015; Rideau Batista Novais, Glia, 2016), then injected with low doses of interleukin1B (20µg/kg) at P1, for the P4 samples. LPD effects were also addressed at birth (P0) Brains cells were dissociated using the Neural Tissue Dissociation Kit of Miltenyi Biotec,in the presence of papain. Then Magnetic Bead coupled antibody (MACS) was used to sort the cells at P4 or at P0. Microglial cells were sorted using the surface marker CD11b. Transcriptome analysis was carried out on this subset of cells, using triplicates of a pool of three different mRNA samples from each experimental group.

2018-12-31 | E-MTAB-6630 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data