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Effects of low protein diet-induced Intra-Uterine Growth Restriction in Rat Whole brain at 28 days, and on microglial brain cells at P4. Double Hit with subclinical doses of IL1B, Rescue by Carbetocin treatment.

ABSTRACT: Female rats were exposed during pregnancy to a normal or low protein diet (as described in Bufffat et al, J. Pathol, 2007 and Zana-Taieb et al, J. Pathol, 2015; Rideau Batista Novais, Glia, 2016), then injected with low doses of interleukin1B (20µg/kg) at P1, for the P4 samples. Brain samples were taken in Trizol at 28 days post-natal for a subset of the animals For the P4 samples, brains cells were dissociated using the Neural Tissue Dissociation Kit of Miltenyi Biotec,in the presence of papain. Then Magnetic Bead coupled antibody (MACS) was used to sort the cells. Microglial cells were sorted using the surface marker CD11b. Transcriptome analysis was carried out on these samples using triplicates of a pool of three different mRNA samples from each experimental group.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Daniel VAIMAN

PROVIDER: E-MTAB-6631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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