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Transition of embryonic stem cells from ground state pluripotency

ABSTRACT: Mouse embryonic stem cells were maintained in minimal and chemically-defined culture conditions supporting naive pluripotency. Inhibitors of the Gsk3 (CHIR99021) and Mek/Erk (PD0325901) pathways were withdrawn, cultures maintained for 24 hours, and subsequently sorted by flow cytometry based on fluorescence of a short-half-life Rex1(Zfp42)::GFP reporter into two populations: Rex1-high cells, functionally capable of reversion to naive pluripotency, and Rex1-low cells that have exited the naive state.

ORGANISM(S): Mus musculus

SUBMITTER: Paul Bertone

PROVIDER: E-MTAB-5304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tracking the embryonic stem cell transition from ground state pluripotency.

Kalkan Tüzer T Olova Nelly N Roode Mila M Mulas Carla C Lee Heather J HJ Nett Isabelle I Marks Hendrik H Walker Rachael R Stunnenberg Hendrik G HG Lilley Kathryn S KS Nichols Jennifer J Reik Wolf W Bertone Paul P Smith Austin A

Development (Cambridge, England) 20170207 7

Mouse embryonic stem (ES) cells are locked into self-renewal by shielding from inductive cues. Release from this ground state in minimal conditions offers a system for delineating developmental progression from naïve pluripotency. Here, we examine the initial transition process. The ES cell population behaves asynchronously. We therefore exploited a short-half-life <i>Rex1::GFP</i> reporter to isolate cells either side of exit from naïve status. Extinction of ES cell identity in single cells is ...[more]

PMID: 28174249

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