Transcription profiling of mouse mesenteric, branchial and axilary lymph nodes from C57Bl/6 femails or CD27 knock out females

ABSTRACT: Mesenteric, brachial and axilary lymph nodes from 4 C57Bl/6 females or 5 CD27KO females, both groups were 6 weeks old, were extracted. The different lymph nodes were pooled and a single cell suspension was created. The cell suspension from WT or CD27KO mice was stained with anti-TCRd-PE (GL3) and anti-CD3e-PE Cy7 (2C-11), and FACSorted for double-positive cells on a BD Aria, obtaining around 1 million cells of each sample. These cells were spun down and the pellet was frozen in liquid nitrogen and kept at -80 ºC before RNA extraction.

ORGANISM(S): Mus musculus

SUBMITTER: Victor Peperzak 

PROVIDER: E-NCMF-23 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress