Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Saccharomyces cerevisiae knock-outs of non-essential chromatin modifiers

ABSTRACT: Non-essential knockouts of chromatin modifiers have been investigated by expression profiling.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Patrick Kemmeren

PROVIDER: E-TABM-1074 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Suppression of Mediator is regulated by Cdk8-dependent Grr1 turnover of the Med3 coactivator.

Gonzalez Deyarina D Hamidi Nurul N Del Sol Ricardo R Benschop Joris J JJ Nancy Thomas T Li Chao C Francis Lewis L Tzouros Manuel M Krijgsveld Jeroen J Holstege Frank C P FC Conlan R Steven RS

Proceedings of the National Academy of Sciences of the United States of America 20140203 7

Mediator, an evolutionary conserved large multisubunit protein complex with a central role in regulating RNA polymerase II-transcribed genes, serves as a molecular switchboard at the interface between DNA binding transcription factors and the general transcription machinery. Mediator subunits include the Cdk8 module, which has both positive and negative effects on activator-dependent transcription through the activity of the cyclin-dependent kinase Cdk8, and the tail module, which is required fo ...[more]

PMID: 24550274

Similar Datasets

Transcription profiling of matching human primary head and neck squamous cell carcinomas and cervical lymph node metastases.

Project description:An experiment was performed to investigate the perservation of gene expression upon metastasis of primary head and neck squamous cell carcinomas to the cervical lymph node.

2006-10-23 | E-TABM-114 | biostudies-arrayexpress

Transcription profiling of wild type vs wild type yeast with controls spiked in different ratios; 1:1, 1:2 and 1:10 respectively

Project description:Wild type versus wildtype Saccharomyces cerevisiae experiment with controls spiked in different ratios; 1:1, 1:2 and 1:10 respectively

2003-04-01 | E-UMCU-1 | biostudies-arrayexpress

Transcription profiling by array of yeast wild type strains compared to a reference pool

Project description:Contains a collection of wildtype Saccharomyces cerevisiae strains for estimating the biological variation. Wildtypes are obtained from the "yeast wildtypes - platereader, wt pool bkg set" HybSet, by randomly taking 100 wt vs. refpool and 100 refpool vs. wt hybridizations.

2011-04-08 | E-TABM-984 | biostudies-arrayexpress

Transcription profiling of Saccharomyces cerevisiae kinase and phosphatase knockouts

Project description:All non-essential protein kinases and phosphatases knockouts have been investigated by expression profiling. To investigate redundancy, double mutants are profiled that show a reduced growth according to synthetic genetic interaction data.

2010-12-11 | E-TABM-907 | biostudies-arrayexpress

Transcriptomics of drougth stress in L. japonicus under normal air conditions

Project description:L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard (University Aarhus, Denmark) and then self-propagated at the University of Seville. Seeds were scarified and surface-sterilized, germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture of vermiculite and sand as solid support. Five seedlings were planted in each pot and grown during 35 days in a growth chamber under 16/8 hours day/night, 20/18M-oM-?M-=C, with a photosynthetic photon flux density of 250 M-oM-?M-=mol/m2M-oM-?M-=s and a constant humidity of 70%. Plants were watered with Hornum nutrient solution. Drought was applied withholding irrigation for the reported period of time and sample plants or leaves were harvested for further analysis.

2012-10-06 | E-MEXP-3710 | biostudies-arrayexpress

Transcription profiling of yeast cells grown into stationary phase compared with exponential mid-log grown cells including a dye-swap

Project description:S. cerevisiae cells grown into stationary phase were compared with exponential mid-log grown cells including a dye-swap. External normalization controls were added in equal amounts to equivalent amounts of total RNA.

2003-04-01 | E-UMCU-4 | biostudies-arrayexpress

Transcription profiling of yeast wild type and YJL103C deletion mutant during glucose starvation in a time series

Project description:Expression profiling of YJL103C deletion mutant compared to wild type during glucose starvation (4h, exponential phase; 24h, diauxic shift; 72h, post-diauxic growth; 144h, entry into stationary phase)

2008-01-01 | E-MEXP-769 | biostudies-arrayexpress

Transcription profiling by array of Lotus japonicus leaves from wild type and Ljgln2-2 mutant plants exposed to drought.

Project description:L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard (University Aarhus, Denmark) and then self-propagated at the University of Seville. Ljgln2-2 mutant was isolated from photorespiratory mutant screening as described previously (Orea et al., 2002; Mï¿½rquez et al., 2005; Betti et al., 2006). The mutant progeny of two consecutive back-crosses with WT was used for the present work. WT and mutant seeds were scarified and surface-sterilized, germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture of vermiculite and sand as solid support. Five seedlings were planted in each pot and grown during 35 days in a growth chamber under 16/8 hours day/night, 20/18ï¿½C, with a photosynthetic photon flux density of 250 ï¿½mol/m2ï¿½s and a constant humidity of 70%. CO2 was automatically injected to a final concentration of 0.7% (v/v), to allow for normal growth of Ljgln2-2 mutant in a photorespiration suppressed atmosphere. Plants were watered with Hornum nutrient solution (Handberg & Stougaard, 1992). Drought was applied withholding irrigation for the reported period of time and sample plants or leaves were harvested for further analysis.

2011-04-29 | E-MEXP-2690 | biostudies-arrayexpress

Transcription profiling of yeast after a shift from media containing raffinose to one containg galactose

Project description:expression profile from a shift from raffinose to galatose. This serves as a control expirement.

2009-09-19 | E-TABM-693 | biostudies-arrayexpress

Transcription profiling of heptatocytes from mice given a high-copper diet

Project description:In order to study the physiological consequences of a high-copper diet on hepatic gene expression, 6 mM CuCl2 was added to the drinking water for a period of 1 month. After this period, livers of seven control mice and eight copper-treated mice were isolated and were subjected to microarray analysis and copper measurements. The hepatic gene expression profile of copper-treated mice was compared to non-treated mice using a pooled reference.

2007-06-01 | E-MEXP-745 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data