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Chromatin immunoprecipitation of Drosophila to identify pMad, dTcf, Doc, Pnr and Slp1 proteins binding during embryonic development

ABSTRACT: Genomewide mapping of Drosophila melanogaster pMad, dTcf, Doc, Pnr and Slp1 protein binding during embryonic development. Two consecutive timepoints (4-6 and 6-8 hrs after egg-laying) were assayed in two independent repeats each. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to high density Affymetrix GeneChip Drosophila Tiling 1.0R array.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot

PROVIDER: E-TABM-1184 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A transcription factor collective defines cardiac cell fate and reflects lineage history.

Junion Guillaume G Spivakov Mikhail M Girardot Charles C Braun Martina M Gustafson E Hilary EH Birney Ewan E Furlong Eileen E M EE

Cell 20120201 3

Cell fate decisions are driven through the integration of inductive signals and tissue-specific transcription factors (TFs), although the details on how this information converges in cis remain unclear. Here, we demonstrate that the five genetic components essential for cardiac specification in Drosophila, including the effectors of Wg and Dpp signaling, act as a collective unit to cooperatively regulate heart enhancer activity, both in vivo and in vitro. Their combinatorial binding does not req ...[more]

PMID: 22304916

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