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Chromatin immunoprecipitation to identify Tinman protein binding during embryonic delelopment in D. melanogaster

ABSTRACT: Genomewide mapping of Drosophila Tinman protein binding during embryonic development. Three consecutive timepoints (2-4, 4-6, 6-8 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Tinman protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot 

PROVIDER: E-TABM-558 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A systematic analysis of Tinman function reveals Eya and JAK-STAT signaling as essential regulators of muscle development.

Liu Ya-Hsin YH   Jakobsen Janus S JS   Valentin Guillaume G   Amarantos Ioannis I   Gilmour Darren T DT   Furlong Eileen E M EE  

Developmental cell 20090201 2

Nk-2 proteins are essential developmental regulators from flies to humans. In Drosophila, the family member tinman is the major regulator of cell fate within the dorsal mesoderm, including heart, visceral, and dorsal somatic muscle. To decipher Tinman's direct regulatory role, we performed a time course of ChIP-on-chip experiments, revealing a more prominent role in somatic muscle specification than previously anticipated. Through the combination of transgenic enhancer-reporter assays, colocaliz  ...[more]

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