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Deciphering the antigen specificities of antibodies by clustering their complementarity determining region sequences.

ABSTRACT:

Importance

Determining antigen and epitope specificity is an essential step in the discovery of therapeutic antibodies as well as in the analysis adaptive immune responses to disease or vaccination. Despite extensive efforts, deciphering antigen specificity solely from BCR amino acid sequence remains a challenging task, requiring a combination of experimental and computational approaches. Here, we describe and experimentally validate a simple and straightforward approach for grouping antibodies that share antigen and epitope specificities based on their CDR sequence similarity. This approach allows us to identify the specificities of a large number of antibodies whose antigen targets are unknown, using a small fraction of antibodies with well-annotated binding specificities.

SUBMITTER: Saputri DS

PROVIDER: S-EPMC10734444 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Deciphering the antigen specificities of antibodies by clustering their complementarity determining region sequences.

Saputri Dianita S DS Ismanto Hendra S HS Nugraha Dendi K DK Xu Zichang Z Horiguchi Yasuhiko Y Sakakibara Shuhei S Standley Daron M DM

mSystems 20231117 6

<h4>Importance</h4>Determining antigen and epitope specificity is an essential step in the discovery of therapeutic antibodies as well as in the analysis adaptive immune responses to disease or vaccination. Despite extensive efforts, deciphering antigen specificity solely from BCR amino acid sequence remains a challenging task, requiring a combination of experimental and computational approaches. Here, we describe and experimentally validate a simple and straightforward approach for grouping ant ...[more]

PMID: 37975681

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