Unknown

Dataset Information

0

Recognition of a virus-encoded ligand by a natural killer cell activation receptor.


ABSTRACT: Natural killer (NK) cells express inhibitory and activation receptors that recognize MHC class I-like molecules on target cells. These receptors may be involved in the critical role of NK cells in controlling initial phases of certain viral infections. Indeed, the Ly49H NK cell activation receptor confers in vivo genetic resistance to murine cytomegalovirus (MCMV) infections, but its ligand was previously unknown. Herein, we use heterologous reporter cells to demonstrate that Ly49H recognizes MCMV-infected cells and a ligand encoded by MCMV itself. Exploiting a bioinformatics approach to the MCMV genome, we find at least 11 ORFs for molecules with previously unrecognized features of predicted MHC-like folds and limited MHC sequence homology. We identify one of these, m157, as the ligand for Ly49H. m157 triggers Ly49H-mediated cytotoxicity, and cytokine and chemokine production by freshly isolated NK cells. We hypothesize that the other ORFs with predicted MHC-like folds may be involved in immune evasion or interactions with other NK cell receptors.

SUBMITTER: Smith HR 

PROVIDER: S-EPMC124383 | biostudies-literature | 2002 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Recognition of a virus-encoded ligand by a natural killer cell activation receptor.

Smith Hamish R C HR   Heusel Jonathan W JW   Mehta Indira K IK   Kim Sungjin S   Dorner Brigitte G BG   Naidenko Olga V OV   Iizuka Koho K   Furukawa Hiroshi H   Beckman Diana L DL   Pingel Jeanette T JT   Scalzo Anthony A AA   Fremont Daved H DH   Yokoyama Wayne M WM  

Proceedings of the National Academy of Sciences of the United States of America 20020611 13


Natural killer (NK) cells express inhibitory and activation receptors that recognize MHC class I-like molecules on target cells. These receptors may be involved in the critical role of NK cells in controlling initial phases of certain viral infections. Indeed, the Ly49H NK cell activation receptor confers in vivo genetic resistance to murine cytomegalovirus (MCMV) infections, but its ligand was previously unknown. Herein, we use heterologous reporter cells to demonstrate that Ly49H recognizes MC  ...[more]

Similar Datasets

| S-EPMC2519616 | biostudies-literature
| S-EPMC3100565 | biostudies-literature
| S-EPMC7935321 | biostudies-literature
| S-EPMC4756352 | biostudies-literature
| S-EPMC7456168 | biostudies-literature
| S-EPMC6448830 | biostudies-literature
| S-EPMC7409301 | biostudies-literature
| S-EPMC3223435 | biostudies-literature
| S-EPMC3343337 | biostudies-literature