Unknown

Dataset Information

0

The effect of particle design on cellular internalization pathways.


ABSTRACT: The interaction of particles with cells is known to be strongly influenced by particle size, but little is known about the interdependent role that size, shape, and surface chemistry have on cellular internalization and intracellular trafficking. We report on the internalization of specially designed, monodisperse hydrogel particles into HeLa cells as a function of size, shape, and surface charge. We employ a top-down particle fabrication technique called PRINT that is able to generate uniform populations of organic micro- and nanoparticles with complete control of size, shape, and surface chemistry. Evidence of particle internalization was obtained by using conventional biological techniques and transmission electron microscopy. These findings suggest that HeLa cells readily internalize nonspherical particles with dimensions as large as 3 mum by using several different mechanisms of endocytosis. Moreover, it was found that rod-like particles enjoy an appreciable advantage when it comes to internalization rates, reminiscent of the advantage that many rod-like bacteria have for internalization in nonphagocytic cells.

SUBMITTER: Gratton SE 

PROVIDER: S-EPMC2575324 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

The effect of particle design on cellular internalization pathways.

Gratton Stephanie E A SE   Ropp Patricia A PA   Pohlhaus Patrick D PD   Luft J Christopher JC   Madden Victoria J VJ   Napier Mary E ME   DeSimone Joseph M JM  

Proceedings of the National Academy of Sciences of the United States of America 20080812 33


The interaction of particles with cells is known to be strongly influenced by particle size, but little is known about the interdependent role that size, shape, and surface chemistry have on cellular internalization and intracellular trafficking. We report on the internalization of specially designed, monodisperse hydrogel particles into HeLa cells as a function of size, shape, and surface charge. We employ a top-down particle fabrication technique called PRINT that is able to generate uniform p  ...[more]

Similar Datasets

| S-EPMC3196066 | biostudies-literature
| S-EPMC5441499 | biostudies-literature
| S-EPMC6835553 | biostudies-literature
| S-EPMC2975856 | biostudies-literature
| S-EPMC5214587 | biostudies-literature
| S-EPMC3216674 | biostudies-literature
| S-EPMC2771217 | biostudies-literature
| S-EPMC3685167 | biostudies-literature
| S-EPMC5224471 | biostudies-literature
| S-EPMC9446767 | biostudies-literature