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Structure of the wild-type human BCL6 POZ domain.

ABSTRACT: BCL6 is a transcriptional repressor that is overexpressed in diffuse large B-cell lymphoma and follicular lymphoma. The N-terminal POZ domain of BCL6 interacts with transcriptional corepressors and targeting these associations is a promising therapeutic strategy. Previous structural studies of the BCL6 POZ domain have used a mutant form because of the low solubility of the wild-type recombinant protein. A method for the purification and crystallization of the wild-type BCL6 POZ domain is described and the crystal structure to 2.1 A resolution is reported. This will be relevant for the design of therapeutics that target BCL6 POZ-domain interaction interfaces.

SUBMITTER: Stead MA

PROVIDER: S-EPMC2593701 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

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Structure of the wild-type human BCL6 POZ domain.

Stead Mark A MA Rosbrook Gareth O GO Hadden Jonathan M JM Trinh Chi H CH Carr Stephen B SB Wright Stephanie C SC

Acta crystallographica. Section F, Structural biology and crystallization communications 20081128 Pt 12

BCL6 is a transcriptional repressor that is overexpressed in diffuse large B-cell lymphoma and follicular lymphoma. The N-terminal POZ domain of BCL6 interacts with transcriptional corepressors and targeting these associations is a promising therapeutic strategy. Previous structural studies of the BCL6 POZ domain have used a mutant form because of the low solubility of the wild-type recombinant protein. A method for the purification and crystallization of the wild-type BCL6 POZ domain is describ ...[more]

PMID: 19052359

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Backbone resonance assignment of the BCL6-BTB/POZ domain.

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Structure of the human Nac1 POZ domain.

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The ansamycin antibiotic, rifamycin SV, inhibits BCL6 transcriptional repression and forms a complex with the BCL6-BTB/POZ domain.

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Crystal structure of wild-type and mutant human Ap4A hydrolase.

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Nac1 interacts with the POZ-domain transcription factor, Miz1.

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Structure of a BCOR corepressor peptide in complex with the BCL6 BTB domain dimer.

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