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Applications of isothermal titration calorimetry in biophysical studies of G-quadruplexes.

ABSTRACT: G-quadruplexes are higher-order nucleic acids structures formed by G-rich sequences that are stabilized by tetrads of hydrogen-bonded guanine bases. Recently, there has been growing interest in the study of G-quadruplexes because of their possible involvement in many biological processes. Isothermal titration calorimetry (ITC) has been proven to be a useful tool to study the energetic aspects of G-quadruplex interactions. Particularly, ITC has been applied many times to determine the thermodynamic properties of drug-quadruplex interactions to screening among various drugs and to address drug design. In the present review, we will focus on the ITC studies of G-quadruplex structures and their interaction with proteins and drugs and the most significant results will be discussed.

SUBMITTER: Pagano B

PROVIDER: S-EPMC2738904 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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Applications of isothermal titration calorimetry in biophysical studies of G-quadruplexes.

Pagano Bruno B Mattia Carlo Andrea CA Giancola Concetta C

International journal of molecular sciences 20090702 7

G-quadruplexes are higher-order nucleic acids structures formed by G-rich sequences that are stabilized by tetrads of hydrogen-bonded guanine bases. Recently, there has been growing interest in the study of G-quadruplexes because of their possible involvement in many biological processes. Isothermal titration calorimetry (ITC) has been proven to be a useful tool to study the energetic aspects of G-quadruplex interactions. Particularly, ITC has been applied many times to determine the thermodynam ...[more]

PMID: 19742177

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