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Regulation of ciliary polarity by the APC/C.

ABSTRACT: Planar cell polarity signaling controls a variety of polarized cell behaviors. In multiciliated Xenopus epidermal cells, recruitment of Dishevelled (Dvl) to the basal body and its localization to the center of the ciliary rootlet are required to correctly position the motile cilia. We now report that the anaphase-promoting complex (APC/C) recognizes a D-box motif of Dvl and ubiquitylates Dvl on a highly conserved lysine residue. Inhibition of APC/C function by knockdown of the ANAPC2 subunit disrupts the polarity of motile cilia and alters the directionality of the fluid movement along the epidermis of the Xenopus embryo. Our results suggest that the APC/C activity enables cilia to correctly polarize in Xenopus epidermal cells.

SUBMITTER: Ganner A

PROVIDER: S-EPMC2764926 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature

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Regulation of ciliary polarity by the APC/C.

Ganner Athina A Lienkamp Soeren S Schäfer Tobias T Romaker Daniel D Wegierski Tomasz T Park Tae Joo TJ Spreitzer Stefan S Simons Matias M Gloy Joachim J Kim Emily E Wallingford John B JB Walz Gerd G

Proceedings of the National Academy of Sciences of the United States of America 20091005 42

Planar cell polarity signaling controls a variety of polarized cell behaviors. In multiciliated Xenopus epidermal cells, recruitment of Dishevelled (Dvl) to the basal body and its localization to the center of the ciliary rootlet are required to correctly position the motile cilia. We now report that the anaphase-promoting complex (APC/C) recognizes a D-box motif of Dvl and ubiquitylates Dvl on a highly conserved lysine residue. Inhibition of APC/C function by knockdown of the ANAPC2 subunit dis ...[more]

PMID: 19805045

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