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Human RECQL5 participates in the removal of endogenous DNA damage.

ABSTRACT: Human RECQL5 is a member of the RecQ helicase family, which maintains genome stability via participation in many DNA metabolic processes, including DNA repair. Human cells lacking RECQL5 display chromosomal instability. We find that cells depleted of RECQL5 are sensitive to oxidative stress, accumulate endogenous DNA damage, and increase the cellular poly(ADP-ribosyl)ate response. In contrast to the RECQ helicase family members WRN, BLM, and RECQL4, RECQL5 accumulates at laser-induced single-strand breaks in normal human cells. RECQL5 depletion affects the levels of PARP-1 and XRCC1, and our collective results suggest that RECQL5 modulates and/or directly participates in base excision repair of endogenous DNA damage, thereby promoting chromosome stability in normal human cells.

SUBMITTER: Tadokoro T

PROVIDER: S-EPMC3484104 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Human RECQL5 participates in the removal of endogenous DNA damage.

Tadokoro Takashi T Ramamoorthy Mahesh M Popuri Venkateswarlu V May Alfred A Tian Jingyan J Sykora Peter P Rybanska Ivana I Wilson David M DM Croteau Deborah L DL Bohr Vilhelm A VA

Molecular biology of the cell 20120912 21

Human RECQL5 is a member of the RecQ helicase family, which maintains genome stability via participation in many DNA metabolic processes, including DNA repair. Human cells lacking RECQL5 display chromosomal instability. We find that cells depleted of RECQL5 are sensitive to oxidative stress, accumulate endogenous DNA damage, and increase the cellular poly(ADP-ribosyl)ate response. In contrast to the RECQ helicase family members WRN, BLM, and RECQL4, RECQL5 accumulates at laser-induced single-str ...[more]

PMID: 22973052

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