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Highly parallel transport recordings on a membrane-on-nanopore chip at single molecule resolution.

ABSTRACT: Membrane proteins are prime drug targets as they control the transit of information, ions, and solutes across membranes. Here, we present a membrane-on-nanopore platform to analyze nonelectrogenic channels and transporters that are typically not accessible by electrophysiological methods in a multiplexed manner. The silicon chip contains 250,000 femtoliter cavities, closed by a silicon dioxide top layer with defined nanopores. Lipid vesicles containing membrane proteins of interest are spread onto the nanopore-chip surface. Transport events of ligand-gated channels were recorded at single-molecule resolution by high-parallel fluorescence decoding.

SUBMITTER: Urban M

PROVIDER: S-EPMC3953990 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Highly parallel transport recordings on a membrane-on-nanopore chip at single molecule resolution.

Urban Michael M Kleefen Alexander A Mukherjee Nobina N Seelheim Patrick P Windschiegl Barbara B Vor der Brüggen Marc M Koçer Armagan A Tampé Robert R

Nano letters 20140217 3

Membrane proteins are prime drug targets as they control the transit of information, ions, and solutes across membranes. Here, we present a membrane-on-nanopore platform to analyze nonelectrogenic channels and transporters that are typically not accessible by electrophysiological methods in a multiplexed manner. The silicon chip contains 250,000 femtoliter cavities, closed by a silicon dioxide top layer with defined nanopores. Lipid vesicles containing membrane proteins of interest are spread on ...[more]

PMID: 24524682

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