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Tb(3+)-tRNA for LRET studies of protein synthesis.

ABSTRACT: When suitably labeled bulk tRNAs are transfected into cells they give rise to FRET (fluorescence resonance energy transfer) signals via binding to ribosomes that provide a measure of total protein synthesis. Application of this approach to monitoring rates of specific protein synthesis requires achieving a very high signal-to-noise ratio. Such high ratios may be attainable using LRET (luminescence resonance energy transfer) in place of FRET. Lanthanide complexes containing an antenna chromophore are excellent LRET donors. Here we describe the synthesis of a Phe-tRNA(Phe) labeled with a Tb(3+) complex, denoted Tb(3+)-Phe-tRNA(Phe) that, notwithstanding the bulkiness of the Tb(3+) complex, is active in protein synthesis.

SUBMITTER: Alonso D

PROVIDER: S-EPMC3779508 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

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Tb(3+)-tRNA for LRET studies of protein synthesis.

Alonso Dulce D Liu Wei W Rosenblum Gabriel G Mani Tomoyasu T Goldman Yale E YE Cooperman Barry S BS

Bioconjugate chemistry 20130617 7

When suitably labeled bulk tRNAs are transfected into cells they give rise to FRET (fluorescence resonance energy transfer) signals via binding to ribosomes that provide a measure of total protein synthesis. Application of this approach to monitoring rates of specific protein synthesis requires achieving a very high signal-to-noise ratio. Such high ratios may be attainable using LRET (luminescence resonance energy transfer) in place of FRET. Lanthanide complexes containing an antenna chromophore ...[more]

PMID: 23734598

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