Structural phenotyping of stem cell-derived cardiomyocytes.

ABSTRACT: Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during striated muscle cell development. To test our metrics, we analyzed the localization of the contractile protein ?-actinin in a variety of primary and stem-cell derived cardiomyocytes. Further, we combined these metrics with data mining algorithms to unbiasedly score the phenotypic maturity of human-induced pluripotent stem cell-derived cardiomyocytes.

SUBMITTER: Pasqualini FS 

PROVIDER: S-EPMC4375945 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature