Structural phenotyping of stem cell-derived cardiomyocytes.
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ABSTRACT:
SUBMITTER: Pasqualini FS
PROVIDER: S-EPMC4375945 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Publications
Structural phenotyping of stem cell-derived cardiomyocytes.
Pasqualini Francesco Silvio FS Sheehy Sean Paul SP Agarwal Ashutosh A Aratyn-Schaus Yvonne Y Parker Kevin Kit KK
Stem cell reports 20150226 3
Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during striated muscle cell development. To test our metrics, we analyzed the localization of the contractile protein α-actinin in a variety of primary and stem-cell derived cardiomyocyte ...[more]
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