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Measurement of Total Antioxidant Capacity in Sub-?L Blood Samples Using Craft Paper-based Analytical Devices.

ABSTRACT: Antioxidants play a role in counteracting free radicals and reactive oxygen species and are thought to help prevent or slow the progression of many chronic diseases, such as cancer, diabetes mellitus, cardiovascular disease, and neurodegenerative diseases. Herein we report a simple way to make a colorimetric assay for measuring total antioxidant capacity (TAC) on craft paper-based analytical devices (cPADs) suitable for sub-?L volume blood samples. We incorporated a microfluidic separation mechanism for isolation of plasma from interfering blood cells. The whole diagnostic process, including cPAD construction, plasma sample preparation, assay, and image thresholding analysis, can be completed in fifteen minutes. We applied our approach toward the measurement of TAC in mice that model Huntington's disease (HD), a fatal, neurodegenerative movement disorder. Results revealed that TAC was significantly elevated in R6/2 HD model mice compared to their age-matched wild-type (WT) controls. We expect that this method, carrying a simple, fast, and sensitive assay on low-cost and disposable paper, will meet the potential needs for point-of-care (POC) testing of TAC, as well as other disease biomarkers in blood and other types of bodily fluids where limited volumes of samples are obtainable.

SUBMITTER: Sun M

PROVIDER: S-EPMC5026239 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Measurement of Total Antioxidant Capacity in Sub-μL Blood Samples Using Craft Paper-based Analytical Devices.

Sun Meng M Johnson Michael A MA

RSC advances 20150618 69

Antioxidants play a role in counteracting free radicals and reactive oxygen species and are thought to help prevent or slow the progression of many chronic diseases, such as cancer, diabetes mellitus, cardiovascular disease, and neurodegenerative diseases. Herein we report a simple way to make a colorimetric assay for measuring total antioxidant capacity (TAC) on craft paper-based analytical devices (cPADs) suitable for sub-μL volume blood samples. We incorporated a microfluidic separation mecha ...[more]

PMID: 27642513

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Project description:Numerous fabrication methods have been reported for microfluidic paper-based analytical devices (μPADs) using barrier materials ranging from photoresist to wax. While these methods have been used with wide success, consistently producing small, high-resolution features using materials and methods that are compatible with solvents and surfactants remains a challenge. Two new methods are presented here for generating μPADs with well-defined, high-resolution structures compatible with solvents and surfactant-containing solutions by partially or fully fusing paper with Parafilm® followed by cutting with a CO2 laser cutter. Partial fusion leads to laminated paper (l-paper) while the complete fusion results in infused paper (i-paper). Patterned structures in l-paper were fabricated by selective removal of the paper but not the underlying Parafilm® using a benchtop CO2 laser. Under optimized conditions, a gap as small as 137 ± 22 μm could be generated. Using this approach, a miniaturized paper 384-zone plate, consisting of circular detection elements with a diameter of 1.86 mm, was fabricated in 64 × 43 mm2 area. Furthermore, these ablation-patterned substrates were confirmed to be compatible with surfactant solutions and common organic solvents (methanol, acetonitrile and dimethylformamide), which has been achieved by very few μPAD patterning techniques. Patterns in i-paper were created by completely cutting out zones of the i-paper and then fixing pre-cut paper into these openings similar to the strategy of fitting a jigsaw piece into a puzzle. Upon heating, unmodified paper was readily sealed into these openings due to partial reflow of the paraffin into the paper. This unique and simple bonding method was illustrated by two types of 3D μPADs, a push-on valve and a time-gated flow distributor, without adding adhesive layers. The free-standing jigsaw-patterned sheets showed good structural stability and solution compatibility, which provided a facile alternative method for fabricating complicated μPADs.

Dataset Information

Measurement of Total Antioxidant Capacity in Sub-?L Blood Samples Using Craft Paper-based Analytical Devices.

Publications

Measurement of Total Antioxidant Capacity in Sub-μL Blood Samples Using Craft Paper-based Analytical Devices.

Similar Datasets

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