Unknown

Dataset Information

0

Highly Improved Electrospray Ionization-Mass Spectrometry Detection of G-Quadruplex-Folded Oligonucleotides and Their Complexes with Small Molecules.


ABSTRACT: G-quadruplexes are nucleic acids structures stabilized by physiological concentration of potassium ions. Because low stability G-quadruplexes are hardly detectable by mass spectrometry, we optimized solvent conditions: isopropanol in a triethylamine/hexafluoroisopropanol mixture highly increased G-quadruplex sensitivity with no modification of the physiological G-quadruplex conformation. G-quadruplexes/G-quadruplex-ligand complexes were also correctly detected at concentration as low as 40 nM. Detection of the physiological conformation of G4s and their complexes opens up the possibility to perform high-throughput screening of G-quadruplex ligands for the development of drug molecules effective against critical human diseases.

SUBMITTER: Scalabrin M 

PROVIDER: S-EPMC5588092 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC2725001 | biostudies-literature
| S-EPMC3165113 | biostudies-literature
| S-EPMC3938891 | biostudies-literature
| S-EPMC2680693 | biostudies-literature
| S-EPMC3176668 | biostudies-literature
| S-EPMC9285393 | biostudies-literature
| S-EPMC2077085 | biostudies-literature
| S-EPMC6422691 | biostudies-literature
| S-EPMC10704597 | biostudies-literature
| S-EPMC3259225 | biostudies-literature