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Zika Virus Specific Diagnostic Epitope Discovery.


ABSTRACT: High-density peptide microarrays allow screening of more than six thousand peptides on a single standard microscopy slide. This method can be applied for drug discovery, therapeutic target identification, and developing of diagnostics. Here, we present a protocol to discover specific Zika virus (ZIKV) diagnostic peptides using a high-density peptide microarray. A human serum sample validated for ZIKV infection was incubated with a high-density peptide microarray containing the entire ZIKV protein translated into 3,423 unique 15 linear amino acid (aa) residues with a 14-aa residue overlap printed in duplicate. Staining with different secondary antibodies within the same array, we detected peptides that bind to Immunoglobulin M (IgM) and Immunoglobulin G (IgG) antibodies present in serum. These peptides were selected for further validation experiments. In this protocol, we describe the strategy followed to design, process, and analyze a high-density peptide microarray.

SUBMITTER: Sabalza M 

PROVIDER: S-EPMC5755560 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Zika Virus Specific Diagnostic Epitope Discovery.

Sabalza Maite M   Barber Cheryl A CA   Abrams William R WR   Montagna Richard R   Malamud Daniel D  

Journal of visualized experiments : JoVE 20171212 130


High-density peptide microarrays allow screening of more than six thousand peptides on a single standard microscopy slide. This method can be applied for drug discovery, therapeutic target identification, and developing of diagnostics. Here, we present a protocol to discover specific Zika virus (ZIKV) diagnostic peptides using a high-density peptide microarray. A human serum sample validated for ZIKV infection was incubated with a high-density peptide microarray containing the entire ZIKV protei  ...[more]

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