Ontology highlight
ABSTRACT:
SUBMITTER: Liu X
PROVIDER: S-EPMC6195120 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Liu Xuefeng X Krawczyk Ewa E Suprynowicz Frank A FA Palechor-Ceron Nancy N Yuan Hang H Dakic Aleksandra A Simic Vera V Zheng Yun-Ling YL Sripadhan Praathibha P Chen Chen C Lu Jie J Hou Tung-Wei TW Choudhury Sujata S Kallakury Bhaskar B Tang Dean G DG Darling Thomas T Thangapazham Rajesh R Timofeeva Olga O Dritschilo Anatoly A Randell Scott H SH Albanese Christopher C Agarwal Seema S Schlegel Richard R
Nature protocols 20170126 2
Historically, it has been difficult to propagate cells in vitro that are derived directly from human tumors or healthy tissue. However, in vitro preclinical models are essential tools for both the study of basic cancer biology and the promotion of translational research, including drug discovery and drug target identification. This protocol describes conditional reprogramming (CR), which involves coculture of irradiated mouse fibroblast feeder cells with normal and tumor human epithelial cells i ...[more]