Reduced expression of DNA repair genes and chemosensitivity in 1p19q codeleted lower-grade gliomas.

ABSTRACT: BACKGROUND:Lower-grade gliomas (LGGs, defined as WHO grades II and III) with 1p19q codeletion have increased chemosensitivity when compared to LGGs without 1p19q codeletion, but the mechanism is currently unknown. METHODS:RNAseq data from 515 LGG patients in the Cancer Genome Atlas (TCGA) were analyzed to compare the effect of expression of the 9 DNA repair genes located on chromosome arms 1p and 19q on progression free survival (PFS) and overall survival (OS) between patients who received chemotherapy and those who did not. Chemosensitivity of cells with DNA repair genes knocked down was tested using MTS cell proliferation assay in HS683 cell line and U251 cell line. RESULTS:The expression of 9 DNA repair genes on 1p and 19q was significantly lower in 1p19q-codeleted tumors (n?=?175) than in tumors without the codeletion (n?=?337) (p?

SUBMITTER: Tang L 

PROVIDER: S-EPMC6697086 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature