Browse
Submit Data
Databases
API
Help

Dataset Information

24 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Promoting P450 BM3 heme domain dimerization with a tris(5-iodoacetamido-1,10-phenanthroline)Ru(II) complex.

ABSTRACT: Protein dimerization often occurs in many biological systems as to provide structural and functional advantages. A tris(5-iodoacetamido-1,10-phenanthroline)Ruthenium(II) complex was shown to promote the covalent dimerization of a P450 BM3 heme domain mutant containing a surface exposed non-native single cysteine residue. The formation of homodimeric species was confirmed by protein gel electrophoresis, mass spectrometry and UV-Vis spectroscopy. The dimeric species could be separated from the monomer and aggregates by size-exclusion chromatography. Docking simulation reveals a plausible structure with two proteins covalently conjugated to the inorganic compound.

SUBMITTER: Kato M

PROVIDER: S-EPMC7769117 | biostudies-literature | 2020 Jul-Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Promoting P450 BM3 heme domain dimerization with a tris(5-iodoacetamido-1,10-phenanthroline)Ru(II) complex.

Kato Mallory M Foley Bridget B Vu Julia J Huynh Michael M Lucero Kathreena K Harmon Caroline C Cheruzel Lionel L

Biotechnology and applied biochemistry 20200617 4

Protein dimerization often occurs in many biological systems as to provide structural and functional advantages. A tris(5-iodoacetamido-1,10-phenanthroline)Ruthenium(II) complex was shown to promote the covalent dimerization of a P450 BM3 heme domain mutant containing a surface exposed non-native single cysteine residue. The formation of homodimeric species was confirmed by protein gel electrophoresis, mass spectrometry and UV-Vis spectroscopy. The dimeric species could be separated from the mon ...[more]

PMID: 33376255

Similar Datasets

Tris[tris-(1,10-phenanthroline-?N,N')iron(II)] dodeca-tungstoferrate dihydrate.

Project description:The title compound, [Fe(C(12)H(8)N(2))(3)](3)[FeW(12)O(40)]·2H(2)O, was prepared under hydro-thermal conditions. The discrete Keggin-type [FeW(12)O(40)](6-) heteropolyoxoanion has threefold symmetry, with the Fe(II) atom located on the threefold rotation axis. The central FeO(4) tetra-hedron in the anion shares its O atoms with four W(3)O(13) trinuclear units, each of which is made up of three edge-shared WO(6) octa-hedral units. The Fe(II) atom in the complex cation, viz [Fe(phen)(3)](2+) (phen is 1,10-phen-anthroline), shows a slightly distorted octa-hedral geometry defined by six N atoms from three phen ligands. The polyoxoanions pack together with the cations, with the disordered water mol-ecules located in voids; the site occupancy factor for each water O atom is 0.33.

| S-EPMC2961299 | biostudies-literature

Tris(1,10-phenanthroline)nickel(II) dichromate tetra-hydrate.

Project description:The asymmetric unit of the title compound, [Ni(C(12)H(8)N(2))(3)][Cr(2)O(7)]·4H(2)O, contains one cation, one anion and four water mol-ecules, three of which are disordered over two sites with equal occupancies. In the cation, the metal centre is coordinated by six N atoms from three 1,10-phenanthroline ligands in a distorted octa-hedral geometry. The [Cr(2)O(7)](2-) anion exhibits a staggered conformation. The crystal packing is stabilized by inter-molecular O-H?O hydrogen bonds and ?-? inter-actions, evidenced by short distances of 3.531?(5)?Å between the centroids of aromatic rings.

| S-EPMC2979795 | biostudies-literature

Tris(1,10-phenanthroline)cobalt(II) bis-(trichloro-acetate).

Project description:In the title complex, [Co(C(12)H(8)N(2))(3)](C(2)Cl(3)O(2))(2), the Co(II) ion lies on a twofold rotation axis and is coordinated by six N atoms from three bis-chelating 1,10-phenanthroline ligands in a distorted octa-hedral environment. The crystal structure is stabilized by weak inter-molecular C-H⋯O hydrogen bonds.

| S-EPMC3152094 | biostudies-literature

Bis[tris-(1,10-phenanthroline)nickel(II)] tris-[dicyanidoargentate(I)] nitrate 4.2-hydrate.

Project description:The title compound, [Ni(C(12)H(8)N(2))(3)](2)[Ag(CN)(2)](3)(NO(3))·4.2H(2)O, crystallizes with two independent [Ni(phen)(3)](2+) cations (phen is 1,10-phenanthroline; both Ni atoms have threefold symmetry and N(6) donor sets), three near-linear [Ag(CN)(2)](-) anions, one nitrate anion (N site symmetry 3) and 4.2 water mol-ecules of crystallization, some of which are disordered. The [Ag(CN)(2)](-) anions are situated within cavities created by the phenanthroline ligands of adjacent [Ni(phen)(3)](2+) cations. Some short C-H⋯O and C-H⋯N inter-actions may help to establish the packing.

| S-EPMC2959559 | biostudies-literature

Tris(1,10-phenanthroline-?(2)N,N')iron(II) bis-[(1,10-phenanthroline-?(2)N,N')tetra-kis-(thio-cyanato-?N)chromate(III)] acetonitrile tris-olvate monohydrate.

Project description:Single crystals of the title heterometallic compound, [Fe(C(12)H(8)N(2))(3)][Cr(NCS)(4)(C(12)H(8)N(2))](2)·3CH(3)CN·H(2)O or [Fe(Cphen)(3)][Cr(NCS)(4)(phen)](2)·3CH(3)CN·H(2)O, were pre-pared using the one-pot open-air reaction of iron powder, Reineckes salt and 1,10-phenanthroline (phen) in acetonitrile. The asymetric unit consists of an [Fe(phen)(3)](2+) cation, two [Cr(phen)(NCS)(4)](-) anions, three acetonitrile solvent mol-ecules and a water mol-ecule. The Fe and Cr atoms both show a slightly distorted octa-hedral FeN(6) and CrN(6) coordination geometry with adjacent angles in the range 79.67?(12)-95.21?(12)°. No classical hydrogen bonding involving the water molecule is observed.

| S-EPMC3344293 | biostudies-literature

Tris(1,10-phenanthroline-?N,N')nickel(II) dinitrate tetra-hydrate.

Project description:In the title complex, [Ni(C(12)H(8)N(2))(3)](NO(3))(2)·4H(2)O, the Ni(II) ion is octa-hedrally coordinated by three bidentate 1,10-phenanthroline ligands, each forming a five-membered chelate ring. In the crystal, O-H?O and C-H?O hydrogen bonds are present between the complex cations, nitrate anions and water mol-ecules. O-H?O hydrogen bonds between the uncoord-inated water mol-ecules lead to the formation of a four-membered ring water cluster, with a planar configuration. There were an additional five grossly disordered water mol-ecules in the asymmetric unit, which were removed by the subroutine SQUEEZE; these were were excluded in the calculation of the molecular weight, etc. ?-? stacking inter-actions between the aromatic rings are also observed [centroid-centroid distances = 3.697?(2), 3.728?(2) and 3.761?(2)?Å].

| S-EPMC3238704 | biostudies-literature

Tris(nitrato-κO,O')bis-(1,10-phenanthroline-κN,N')holmium(III).

Project description:In the title compound, [Ho(NO(3))(3)(C(12)H(8)N(2))(2)], the ten-coordinate Ho(III) ion is chelated by four N atoms from two phenanthroline (phen) ligands and six O atoms from three bidentate nitrate groups. The environment around the Ho atom can be described as a distorted bicapped square anti-prism. Two phenanthroline ligands form a dihedral angle of 43.72 (13)°. Short inter-molecular distances between the centroids of the six-membered rings [3.6887 (14)-3.8374 (16) Å] indicate the existence of π-π inter-actions, which link the mol-ecules into stacks extended in the [10] direction. The crystal packing is further stabilized by weak inter-molecular C-H⋯O hydrogen bonds.

| S-EPMC2961938 | biostudies-literature

Bis(2-chloro-1,10-phenanthroline-κN,N')(thio-cyanato-κN)zinc (2-chloro-1,10-phenanthroline-κN,N')tris-(thio-cyanato-κN)zincate.

Project description:The asymmetric unit of the title compound, [Zn(NCS)(C(12)H(7)ClN(2))(2)][Zn(NCS)(3)(C(12)H(7)ClN(2))], contains two cations and two anions. In the cations, the Zn(II) ions have distorted trigonal-bipyramidal environments formed by four N atoms from two 2-chloro-1,10-phenanthroline (cphen) ligands and one N atom from a thio-cyanate ligand. The Zn(II) atoms in the complex anions also have distorted trigonal-bipyramidal environments, formed by two N atoms from a cphen ligand and three N atoms from three thio-cyanato ligands. The crystal packing exhibits π-π inter-actions between the rings of the cphen ligands [shortest centroid-centroid distance = 3.586 (5) Å] and short inter-molecular S⋯Cl [3.395 (5) Å] and S⋯S [3.440 (4) Å] contacts.

| S-EPMC3274921 | biostudies-literature

Tris(1,10-phenanthroline-5,6-dione-?N,N')zinc bis-(perchlorate) acetonitrile monosolvate.

Project description:In the title compound, [Zn(C(12)H(6)N(2)O(2))(3)](ClO(4))(2)·CH(3)CN, the Zn(II) atom is coordinated by six N atoms from three chelating 1,10-phenanthroline-5,6-dione ligands in a distorted octa-hedral environment. In the crystal, inter-molecular C-H?O hydrogen bonds and O?? and N?? inter-actions [O?centroid distances = 2.907?(5) and 2.843?(7)?Å; N?centroid distance = 2.861?(10)?Å] link the complex cations, perchlorate anions and acetonitrile solvent mol-ecules into a three-dimensional network.

| S-EPMC3201451 | biostudies-literature

Tris(1,10-phenanthroline)cobalt(II) bis-(perrhenate) monohydrate.

Project description:In the title compound, [Co(C(12)H(8)N(2))(3)][ReO(4)](2)·H(2)O, the Co(II) atom is coordinated by three 1,10-phenanthroline ligands in a distorted octa-hedral arrangement. In the crystal, the components are linked by O-H?O, C-H?O and aromatic ?-? stacking [shortest centroid-centroid separation = 3.659?(5)?Å] inter-actions.

| S-EPMC3006859 | biostudies-other

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data