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Data-deposition protocols for correlative soft X-ray tomography and super-resolution structured illumination microscopy applications.

ABSTRACT: This protocol illustrates the steps necessary to deposit correlated 3D cryo-imaging data from cryo-structured illumination microscopy and cryo-soft X-ray tomography with the BioStudies and EMPIAR deposition databases of the European Bioinformatics Institute. There is currently a real need for a robust method of data deposition to ensure unhindered access to and independent validation of correlative light and X-ray microscopy data to allow use in further comparative studies, educational activities, and data mining. For complete details on the use and execution of this protocol, please refer to Kounatidis et al. (2020).

SUBMITTER: Iudin A

PROVIDER: S-EPMC7811169 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Data-deposition protocols for correlative soft X-ray tomography and super-resolution structured illumination microscopy applications.

Iudin Andrii A Sarkans Ugis U Koronfel Mohamed A MA Fish Tom T Dobbie Ian I Harkiolaki Maria M Patwardhan Ardan A Kleywegt Gerard J GJ

STAR protocols 20210113 1

This protocol illustrates the steps necessary to deposit correlated 3D cryo-imaging data from cryo-structured illumination microscopy and cryo-soft X-ray tomography with the BioStudies and EMPIAR deposition databases of the European Bioinformatics Institute. There is currently a real need for a robust method of data deposition to ensure unhindered access to and independent validation of correlative light and X-ray microscopy data to allow use in further comparative studies, educational activitie ...[more]

PMID: 33490973

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