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Site-specific chemical fatty-acylation for gain-of-function analysis of protein S-palmitoylation in live cells.

ABSTRACT: Protein S-palmitoylation, or S-fatty-acylation, regulates many fundamental cellular processes in eukaryotes. Herein, we present a chemical fatty-acylation approach that involves site-specific incorporation of cycloalkyne-containing unnatural amino acids and subsequent bioorthogonal reactions with fatty-acyl tetrazines to install fatty-acylation mimics at target protein sites, allowing gain-of-function analysis of S-palmitoylation in live cells.

SUBMITTER: Li Y

PROVIDER: S-EPMC8177487 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Site-specific chemical fatty-acylation for gain-of-function analysis of protein S-palmitoylation in live cells.

Li Yumeng Y Wang Shushu S Chen Yanchi Y Li Manjia M Dong Xiaoshu X Hang Howard C HC Peng Tao T

Chemical communications (Cambridge, England) 20201023 89

Protein S-palmitoylation, or S-fatty-acylation, regulates many fundamental cellular processes in eukaryotes. Herein, we present a chemical fatty-acylation approach that involves site-specific incorporation of cycloalkyne-containing unnatural amino acids and subsequent bioorthogonal reactions with fatty-acyl tetrazines to install fatty-acylation mimics at target protein sites, allowing gain-of-function analysis of S-palmitoylation in live cells. ...[more]

PMID: 33094750

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