Scanning electron diffraction data collected from peptide microcrystals
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ABSTRACT:
SUBMITTER: Marcus Gallagher-Jones
PROVIDER: EMPIAR-10231 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Nanoscale mosaicity revealed in peptide microcrystals by scanning electron nanodiffraction.
Gallagher-Jones Marcus M Ophus Colin C Bustillo Karen C KC Boyer David R DR Panova Ouliana O Glynn Calina C Zee Chih-Te CT Ciston Jim J Mancia Kevin Canton KC Minor Andrew M AM Rodriguez Jose A JA
Communications biology 20190118
Changes in lattice structure across sub-regions of protein crystals are challenging to assess when relying on whole crystal measurements. Because of this difficulty, macromolecular structure determination from protein micro and nanocrystals requires assumptions of bulk crystallinity and domain block substructure. Here we map lattice structure across micron size areas of cryogenically preserved three-dimensional peptide crystals using a nano-focused electron beam. This approach produces diffracti ...[more]
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