Dataset Information
Single-particle cryo-EM dataset of the complex between a nucleosome and the regulatory linker of ALC1
Ontology highlight
ABSTRACT:
SUBMITTER: Guillaume Gaullier
PROVIDER: EMPIAR-10465 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch.
Cell reports 20201201 12
Upon DNA damage, the ALC1/CHD1L nucleosome remodeling enzyme (remodeler) is activated by binding to poly(ADP-ribose). How activated ALC1 recognizes the nucleosome, as well as how this recognition is coupled to remodeling, is unknown. Here, we show that remodeling by ALC1 requires a wild-type acidic patch on the entry side of the nucleosome. The cryo-electron microscopy structure of a nucleosome-ALC1 linker complex reveals a regulatory linker segment that binds to the acidic patch. Mutations with ...[more]