Ontology highlight
ABSTRACT:
SUBMITTER: Neil James Rzechorzek
PROVIDER: EMPIAR-10472 | biostudies-other |
REPOSITORIES: biostudies-other
Rzechorzek Neil J NJ Hardwick Steven W SW Jatikusumo Vincentius A VA Chirgadze Dimitri Y DY Pellegrini Luca L
Nucleic acids research 20200701 12
DNA unwinding in eukaryotic replication is performed by the Cdc45-MCM-GINS (CMG) helicase. Although the CMG architecture has been elucidated, its mechanism of DNA unwinding and replisome interactions remain poorly understood. Here we report the cryoEM structure at 3.3 Å of human CMG bound to fork DNA and the ATP-analogue ATPγS. Eleven nucleotides of single-stranded (ss) DNA are bound within the C-tier of MCM2-7 AAA+ ATPase domains. All MCM subunits contact DNA, from MCM2 at the 5'-end to MCM5 at ...[more]